本文選題：辣椒 + BiP基因�。� 參考：《西北農(nóng)林科技大學(xué)》2017年碩士論文

【摘要】：各種生長逆境造成的植物體蛋白結(jié)構(gòu)的破壞和錯誤折疊,嚴(yán)重影響著植物的生長發(fā)育。BiP蛋白因為能夠緩解由錯誤折疊蛋白的積累導(dǎo)致的內(nèi)質(zhì)網(wǎng)脅迫,在植物抵抗逆境過程中發(fā)揮著重要作用。同時,Bi P作為分子伴侶參與到內(nèi)質(zhì)網(wǎng)脅迫所激發(fā)的未折疊蛋白響應(yīng)(UPR)進(jìn)程,多個研究認(rèn)為其參與到了抵抗蛋白錯誤折疊的內(nèi)質(zhì)網(wǎng)保護(hù)機制中。在本研究中,我們分析了三個辣椒Bi P基因(CaBiP1,CaBiP2和CaBiP3)的表達(dá)模式以及氨基酸序列結(jié)構(gòu)。結(jié)果顯示了三個基因的氨基酸序列擁有BiP蛋白所必需的保守功能域,但是在保守基序motif和內(nèi)含子外顯子分布方面,CaBiP3相對于CaBiP1和CaBiP2具有部分的差異。正常和脅迫條件下,CaBiP1和CaBiP2的表達(dá)模式說明它們屬于組成性基因;而CaBiP3主要在特定的脅迫條件下表達(dá),屬于誘導(dǎo)型基因。CaBiP1的過表達(dá)加強了轉(zhuǎn)基因擬南芥的抗非生物脅迫能力。非生物脅迫下轉(zhuǎn)基因植株相對更高的發(fā)芽率、根長、成活率、可溶性蛋白含量以及失水率的降低,未折疊蛋白途徑被激活均證明了抗脅迫能力的增強。病毒誘導(dǎo)的基因沉默(VIGS)在轉(zhuǎn)錄水平上大大降低CaBiP1的表達(dá),降低了辣椒抵抗逆境脅迫(熱脅迫、鹽脅迫和滲透脅迫)以及二硫蘇糖醇(DTT)引發(fā)的內(nèi)質(zhì)網(wǎng)脅迫的能力。這些結(jié)果由沉默植株葉片中增加的H2O2積累量、丙二醛(MDA)含量、相對電導(dǎo)率(REL)、失水率以及降低的可溶性蛋白含量得以證明。我們的結(jié)果為CaBiP家族基因在不同調(diào)節(jié)機制中功能的進(jìn)一步研究奠定了基礎(chǔ),以及證明了CaBiP1基因在辣椒植株在應(yīng)對非生物脅迫過程中發(fā)揮重要作用。
[Abstract]:The destruction and misfolding of plant protein structure caused by various growth stresses seriously affect the growth and development of plants. BiP protein can alleviate the endoplasmic reticulum stress caused by the accumulation of misfolded proteins. It plays an important role in plant resistance to stress. At the same time, BiP, as a molecular chaperone, is involved in the UPR-responsive process of unfolded proteins stimulated by endoplasmic reticulum stress, which is considered to be involved in the protection mechanism of endoplasmic reticulum against protein misfolding. In this study, we analyzed the expression patterns and amino acid sequence structure of three Pepper BiP genes, CaBiP1, CaBiP2 and CaBiP3. The results showed that the amino acid sequences of the three genes possessed the conserved functional domains necessary for the bip protein, but there were some differences between CaBiP3 and CaBiP1 and CaBiP2 in the distribution of conserved motifs motif and intron exons. The expression patterns of CaBiP1 and CaBiP2 under normal and stress conditions indicated that they belonged to constitutive genes, while CaBiP3 was mainly expressed under specific stress conditions, and the overexpression of the inducible gene .CaBiP1 enhanced the ability of transgenic Arabidopsis thaliana to resist abiotic stress. The relative higher germination rate, root length, survival rate, soluble protein content and water loss rate of transgenic plants under abiotic stress, and the activation of unfolded protein pathway proved the enhancement of resistance to stress. Virus-induced gene silencing significantly reduced the expression of CaBiP1 at the transcriptional level, and reduced the ability of capsicum to resist stress induced by heat, salt and osmotic stress, as well as endoplasmic reticulum stress induced by dithiothreitol (DTT). These results were demonstrated by the increased H _ 2O _ 2 accumulation in leaves of silencing plants, the content of malondialdehyde (MDA) MDAs, the relative electrical conductivity (REC), the water loss rate and the reduced soluble protein content. Our results laid a foundation for the further study of the function of CaBiP family genes in different regulatory mechanisms and demonstrated that CaBiP1 gene plays an important role in the response of pepper plants to abiotic stress.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：S641.3

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