本文選題：三七總皂苷 + 腦出血�。� 參考：《暨南大學(xué)學(xué)報(bào)(自然科學(xué)與醫(yī)學(xué)版)》2017年05期

【摘要】：目的:研究三七總皂苷對(duì)腦出血大鼠神經(jīng)細(xì)胞凋亡及相關(guān)基因的影響.方法:將大鼠隨機(jī)分為假手術(shù)組、模型組和三七總皂苷組,利用膠原酶注射尾狀核法制作腦出血模型,利用免疫組化染色法及細(xì)胞凋亡染色法檢測(cè)各組大鼠Bcl-2、Bax、Caspase-3及TUNEL陽性細(xì)胞數(shù)目.結(jié)果:在6 h、2 d、3 d、5 d、7 d共5個(gè)時(shí)間點(diǎn),模型組、三七總皂苷組大鼠神經(jīng)細(xì)胞凋亡情況、Bcl-2、Bax、Caspase-3與假手術(shù)組相比差異均顯著,具有統(tǒng)計(jì)學(xué)意義(P0.05);三七總皂苷組大鼠神經(jīng)細(xì)胞凋亡情況、Bcl-2、Bax、Caspase-3與模型組相比差異均顯著,具有統(tǒng)計(jì)學(xué)意義(P0.05).結(jié)論:三七總皂苷治療可以顯著下調(diào)Bax、Caspase-3的表達(dá)水平,提高Bcl-2的表達(dá)水平,減少神經(jīng)細(xì)胞凋亡,降低腦出血后繼發(fā)性損傷.
[Abstract]:Aim: to study the effect of Panax notoginseng saponins on neuronal apoptosis and related genes in rats with intracerebral hemorrhage. Methods: rats were randomly divided into sham-operation group, model group and panax notoginseng saponins group. Intracerebral hemorrhage model was made by injection of collagenase into caudate nucleus. Immunohistochemical staining and apoptosis staining were used to detect the number of Caspase-3 and Tunel positive cells. Results: the apoptosis of nerve cells in model group, panax notoginseng saponins group and sham operation group were significantly different from those in sham operation group at 6 h, 2 d, 3 d, 5 d and 7 d, and the apoptosis of nerve cells in model group and panax notoginseng saponins group were significantly higher than those in sham operation group. The apoptosis of nerve cells in the panax notoginseng saponins group was significantly different from that in the model group. Conclusion: panax notoginseng saponins can significantly down-regulate the expression of Caspase-3, increase the expression of Bcl-2, reduce neuronal apoptosis and reduce the secondary injury after intracerebral hemorrhage.
【作者單位】： 廣東藥科大學(xué)藥學(xué)院;暨南大學(xué)附屬第一醫(yī)院藥學(xué)部;
【基金】：廣東省自然科學(xué)基金項(xiàng)目(2015A030313580)
【分類號(hào)】：R285.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 魏鳳玲,朱春波,朱立平,李培紅;三七總皂苷提取工藝優(yōu)選[J];中國中藥雜志;2000年12期

2 索建蘭;沈峰;米海林;;三七總皂苷提取工藝的研究[J];藥物分析雜志;2011年06期

3 黃天衛(wèi);張文斌;孫玉琴;韋美麗;楊建忠;;不同輪作年限三七總皂苷含量分析[J];現(xiàn)代中藥研究與實(shí)踐;2011年03期

4 韓一波;三七總皂苷的研究進(jìn)展[J];中國藥師;2005年03期

5 付開禮;;三七總皂苷在骨科的藥理與臨床研究進(jìn)展[J];云南中醫(yī)中藥雜志;2006年02期

6 任周新;李麗;李君;羅永明;;黃芪總皂苷和三七總皂苷配伍對(duì)大鼠局灶性腦缺血的影響[J];中國實(shí)驗(yàn)方劑學(xué)雜志;2006年12期

7 洪思佳;萬建波;張慶文;張q，

本文編號(hào)：2033467