本文選題：雞 + 脂類代謝��； 參考：《畜牧獸醫(yī)學(xué)報(bào)》2017年02期

【摘要】：本研究旨在探討雞MOGAT和DGAT基因的表達(dá)和調(diào)控特性。首先克隆了具有MOGAT活性的MOGAT1、MOGAT2、LPGAT1和具有DGAT活性的DGAT2和SOAT1對(duì)應(yīng)基因的編碼區(qū)序列,并對(duì)其組織表達(dá)特性進(jìn)行了研究;其次選取產(chǎn)蛋前期母雞和產(chǎn)蛋高峰期母雞作為試驗(yàn)材料,利用實(shí)時(shí)熒光定量PCR技術(shù)分析不同生理時(shí)期雞肝中這些基因的表達(dá)變化規(guī)律;在此基礎(chǔ)上利用雌激素處理的體內(nèi)、體外試驗(yàn),分析這些基因的表達(dá)調(diào)控機(jī)制。結(jié)果表明,雞MOGAT1、MOGAT2、LPGAT1、DGAT2和SOAT1基因在各組織中表現(xiàn)出廣泛表達(dá)的特性,并在脂類代謝旺盛的器官如肝、腎、小腸(十二指腸、空腸、回腸)等器官中表達(dá)較高,但DGAT2基因在雞肝中的表達(dá)量較低,而MOGAT2基因主要在雞小腸組織(十二指腸、空腸、回腸)中表達(dá),MOGAT1在除胰腺外的大部分組織中都有表達(dá);產(chǎn)蛋高峰期母雞肝中MOGAT1、LPGAT1和SOAT1的表達(dá)量均顯著低于產(chǎn)蛋前期母雞;經(jīng)不同濃度雌激素處理后,這些基因在雞肝、十二指腸、腎以及雞胚肝原代細(xì)胞中的表達(dá)量維持不變或顯著下調(diào)。綜上所述,已知的甘油三酯合成的單酰甘油通路中相關(guān)基因不是雞肝TG合成代謝中的限速基因,在雌激素誘導(dǎo)的雞TG合成代謝中,單酰甘油通路不發(fā)揮主導(dǎo)作用。
[Abstract]:The purpose of this study was to investigate the expression and regulatory characteristics of MOGAT and DGAT genes in chickens. The coding region sequences of MOGAT active MOGAT1 / MOGAT2 LPGAT1 and DGAT active DGAT2 and SOAT1 corresponding genes were cloned, and their tissue expression characteristics were studied. Secondly, early laying hens and laying peak hens were selected as experimental materials. The expression of these genes in chicken liver at different physiological stages was analyzed by real-time fluorescence quantitative PCR, and the regulation mechanism of these genes was analyzed by estrogen treatment in vivo and in vitro. The results showed that the genes of MOGAT1, MOGAT2, LPGAT1, DGAT2 and SOAT1 were widely expressed in various tissues, and were highly expressed in organs with high lipid metabolism, such as liver, kidney, small intestine (duodenum, jejunum, ileum) and so on. However, the expression of DGAT2 gene in chicken liver was low, while MOGAT2 gene was mainly expressed in small intestine (duodenum, jejunum, ileum) of chicken. The expression of MOGAT1 LPGAT1 and SOAT1 in the liver of hens during peak laying period was significantly lower than that in prelaying hens, and these genes were treated with different concentrations of estrogen in the liver and duodenum of chickens. The expression of renal and chicken embryonic liver primary cells remained unchanged or significantly down-regulated. In conclusion, the known genes related to triglyceride synthesis are not rate-limiting genes in TG synthesis metabolism of chicken liver, but not in estrogen-induced TG synthesis metabolism.
【作者單位】： 河南農(nóng)業(yè)大學(xué)牧醫(yī)工程學(xué)院河南省家禽種質(zhì)資源創(chuàng)新工程研究中心河南省家禽育種國(guó)際聯(lián)合實(shí)驗(yàn)室;
【基金】：河南省國(guó)際合作項(xiàng)目(162102410030) 國(guó)家自然科學(xué)基金(31372330) 農(nóng)業(yè)部現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系(CARS-41-K04) 河南省重大科技攻關(guān)計(jì)劃項(xiàng)目(112101110800)
【分類號(hào)】：S831.2
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本文編號(hào)：2021806