本文選題：雞 + 脂類代謝�。� 參考：《畜牧獸醫(yī)學報》2017年02期

【摘要】：本研究旨在探討雞MOGAT和DGAT基因的表達和調控特性。首先克隆了具有MOGAT活性的MOGAT1、MOGAT2、LPGAT1和具有DGAT活性的DGAT2和SOAT1對應基因的編碼區(qū)序列,并對其組織表達特性進行了研究;其次選取產(chǎn)蛋前期母雞和產(chǎn)蛋高峰期母雞作為試驗材料,利用實時熒光定量PCR技術分析不同生理時期雞肝中這些基因的表達變化規(guī)律;在此基礎上利用雌激素處理的體內(nèi)、體外試驗,分析這些基因的表達調控機制。結果表明,雞MOGAT1、MOGAT2、LPGAT1、DGAT2和SOAT1基因在各組織中表現(xiàn)出廣泛表達的特性,并在脂類代謝旺盛的器官如肝、腎、小腸(十二指腸、空腸、回腸)等器官中表達較高,但DGAT2基因在雞肝中的表達量較低,而MOGAT2基因主要在雞小腸組織(十二指腸、空腸、回腸)中表達,MOGAT1在除胰腺外的大部分組織中都有表達;產(chǎn)蛋高峰期母雞肝中MOGAT1、LPGAT1和SOAT1的表達量均顯著低于產(chǎn)蛋前期母雞;經(jīng)不同濃度雌激素處理后,這些基因在雞肝、十二指腸、腎以及雞胚肝原代細胞中的表達量維持不變或顯著下調。綜上所述,已知的甘油三酯合成的單酰甘油通路中相關基因不是雞肝TG合成代謝中的限速基因,在雌激素誘導的雞TG合成代謝中,單酰甘油通路不發(fā)揮主導作用。
[Abstract]:The purpose of this study was to investigate the expression and regulatory characteristics of MOGAT and DGAT genes in chickens. The coding region sequences of MOGAT active MOGAT1 / MOGAT2 LPGAT1 and DGAT active DGAT2 and SOAT1 corresponding genes were cloned, and their tissue expression characteristics were studied. Secondly, early laying hens and laying peak hens were selected as experimental materials. The expression of these genes in chicken liver at different physiological stages was analyzed by real-time fluorescence quantitative PCR, and the regulation mechanism of these genes was analyzed by estrogen treatment in vivo and in vitro. The results showed that the genes of MOGAT1, MOGAT2, LPGAT1, DGAT2 and SOAT1 were widely expressed in various tissues, and were highly expressed in organs with high lipid metabolism, such as liver, kidney, small intestine (duodenum, jejunum, ileum) and so on. However, the expression of DGAT2 gene in chicken liver was low, while MOGAT2 gene was mainly expressed in small intestine (duodenum, jejunum, ileum) of chicken. The expression of MOGAT1 LPGAT1 and SOAT1 in the liver of hens during peak laying period was significantly lower than that in prelaying hens, and these genes were treated with different concentrations of estrogen in the liver and duodenum of chickens. The expression of renal and chicken embryonic liver primary cells remained unchanged or significantly down-regulated. In conclusion, the known genes related to triglyceride synthesis are not rate-limiting genes in TG synthesis metabolism of chicken liver, but not in estrogen-induced TG synthesis metabolism.
【作者單位】： 河南農(nóng)業(yè)大學牧醫(yī)工程學院河南省家禽種質資源創(chuàng)新工程研究中心河南省家禽育種國際聯(lián)合實驗室;
【基金】：河南省國際合作項目(162102410030) 國家自然科學基金(31372330) 農(nóng)業(yè)部現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術體系(CARS-41-K04) 河南省重大科技攻關計劃項目(112101110800)
【分類號】：S831.2
，

本文編號：2021806