本文選題：大豆 + 鎘　； 參考：《生態(tài)學(xué)雜志》2016年12期

【摘要】：土壤主要污染物中的重金屬鎘(Cd)已被列為優(yōu)先關(guān)注污染物以及人類致癌物之一,會影響農(nóng)作物的生長發(fā)育,并且可以通過食物鏈進入人體,影響人類健康。已有研究表明,在模式植物擬南芥和水稻中,Nramp蛋白家族在重金屬鎘吸收轉(zhuǎn)運和調(diào)控過程中起到了關(guān)鍵作用,但在大豆中尚未有該基因參與重金屬鎘吸收和轉(zhuǎn)運的報道。通過大豆數(shù)據(jù)庫的分析,發(fā)現(xiàn)了一個Nramp家族基因,命名為Gm Nramp1(Glyma07g02680)。RT-PCR分析結(jié)果表明,在不同時間75μmol·L-1Cd Cl2處理的大豆根、子葉、胚軸和葉中Gm Nramp1基因均下調(diào)表達,而且在Cd處理的根系轉(zhuǎn)錄組測序分析中也得到了相似的結(jié)果。這些結(jié)果說明,Gm Nramp1基因可能在大豆中參與Cd吸收或轉(zhuǎn)運過程。該研究為大豆抗重金屬育種提供理論基礎(chǔ)和基因資源。
[Abstract]:Cadmium and cadmium (CD), a heavy metal in the soil major pollutants, has been listed as one of the priority pollutants and human carcinogens, which will affect the growth and development of crops, and can enter the human body through the food chain and affect human health. Some studies have shown that the Nramp protein family plays a key role in heavy metal uptake and transport in Arabidopsis thaliana and rice. However, there is no report that Nramp protein is involved in heavy metal cadmium absorption and transport in soybean. Through the analysis of soybean database, we found a gene of Nramp family named GM Nramp1 Glyma07g02680. RT-PCR analysis showed that GM Nramp1 gene was down-expressed in root, cotyledon, cotyledon and leaf of soybean treated with 75 渭 mol L -1 CD Cl 2 at different time. Similar results were obtained in CD-treated root transcriptome sequencing. These results suggest that Gm Nramp1 gene may be involved in CD absorption or transport in soybean. This study provides theoretical basis and genetic resources for soybean breeding for heavy metal resistance.
【作者單位】： 中國科學(xué)院沈陽應(yīng)用生態(tài)研究所;中國科學(xué)院大學(xué);
【基金】：國家自然科學(xué)基金項目(31370526和31400336)資助
【分類號】：X53;S565.1

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