本文選題：微囊藻毒素-LR + 草魚(yú)　； 參考：《農(nóng)業(yè)環(huán)境科學(xué)學(xué)報(bào)》2017年12期

【摘要】：為探討微囊藻毒素-LR(microcystin-LR,MC-LR)對(duì)魚(yú)類的免疫毒性,采用急性毒性實(shí)驗(yàn)方法,分別對(duì)草魚(yú)經(jīng)腹腔注射不同劑量的MC-LR并于24、48、72 h和96 h后取免疫器官脾臟和頭腎進(jìn)行組織顯微結(jié)構(gòu)分析。結(jié)果顯示,隨著MC-LR劑量的增加和時(shí)間的延長(zhǎng),脾臟組織呈現(xiàn)出細(xì)胞空泡化并伴隨著黑色素巨噬細(xì)胞先增多后減少的現(xiàn)象,尤其在75μg MC-LR·kg~(-1)BW和100μg MC-LR·kg~(-1)BW劑量組染毒48 h后,黑色素巨噬細(xì)胞中心體積顯著增大;頭腎組織顯微結(jié)構(gòu)變化主要表現(xiàn)為血竇、血管擴(kuò)張,在75μg MC-LR·kg~(-1)BW和100μg MC-LR·kg~(-1)BW劑量組中的草魚(yú)染毒72 h和96 h后,淋巴組織松散、排列混亂,淋巴細(xì)胞空泡化甚至細(xì)胞裂解。此外,采用熒光定量PCR分析了草魚(yú)經(jīng)不同劑量MC-LR處理96 h后脾臟和頭腎中BAFF和APRIL基因表達(dá)的變化。結(jié)果顯示BAFF和APRIL的表達(dá)均被不同程度地抑制,其中脾臟組織中BAFF基因在75μg MC-LR·kg~(-1)BW劑量組中被顯著抑制(P0.05),頭腎組織BAFF基因在75、100μg MC-LR·kg~(-1)BW劑量組中均被顯著抑制(P0.05),而APRIL除了在25μg MC-LR·kg~(-1)BW劑量組的脾臟組織中表達(dá)下調(diào)不顯著外,在其他處理組均被顯著抑制(P0.05)。以上研究結(jié)果表明,MC-LR可導(dǎo)致草魚(yú)免疫器官一系列的病理變化,并有時(shí)間和劑量效應(yīng),此外,MC-LR還可抑制B淋巴細(xì)胞分裂相關(guān)基因的表達(dá)。
[Abstract]:In order to study the immunotoxicity of microcystin-LRN MC-LRR on fish, acute toxicity test was used to analyze the microstructure of spleen and head kidney of immune organs of grass carp after intraperitoneal injection of different doses of MC-LR at 244872 h and 96 h. The results showed that with the increase of MC-LR dose and the prolongation of time, the spleen tissues showed vacuolation and then decreased with the increase of melanin macrophages, especially in 75 渭 g MC-LR Kg-Kg-1 and BW 100 渭 g MC-LR Kg-Kg-1BW groups, 48 h after exposure to MC-LR and 100 渭 g MC-LR, respectively, after 48 hours of exposure to BW100 渭 g MC-LR, the splenic tissues showed vacuolation, followed by a decrease of melanin macrophages. The central volume of melanin macrophages increased significantly, and the microstructures of the head and kidney tissues mainly showed blood sinuses and vasodilation. After exposure to 75 渭 g MC-LR kg ~ (-1) / BW and 100 渭 g M ~ (C-LR) / kg ~ (-1) BW groups for 72 h and 96 h, the lymphatic tissues were loosely arranged and disorganized. Lymphocyte vacuolation and even cell lysis. In addition, the changes of BAFF and APRIL gene expression in spleen and head kidney of grass carp treated with different doses of MC-LR for 96 h were analyzed by fluorescence quantitative PCR. The results showed that the expression of BAFF and APRIL were inhibited to some extent. The expression of BAFF gene in spleen tissue was significantly inhibited in 75 渭 g MC-LR kg-1 BW group, while in head and kidney tissue BAFF gene was significantly inhibited in 75100 渭 g MC-LR kg-1 BW group, but APRIL was not significantly down-regulated in spleen tissue of 25 渭 g MC-LR Kg-1g BW group. In other treatment groups, P0.05 was significantly inhibited. These results suggest that MC-LR can induce a series of pathological changes in the immune organs of grass carp, and in addition, MC-LR can inhibit the expression of mitotic genes in B lymphocytes.
【作者單位】： 江西農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院;江西師范大學(xué)生命學(xué)院;江西農(nóng)業(yè)大學(xué)生物科學(xué)與工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31460146,31260643,31760764)~~
【分類號(hào)】：X503.225
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本文編號(hào)：2004380