發(fā)布時(shí)間：2018-06-06 04:46

  本文選題：CiWRKY + 中間錦雞兒�。� 參考：《分子植物育種》2017年04期

【摘要】：通過RACE技術(shù)從中間錦雞兒中克隆得到一個(gè)WRKY轉(zhuǎn)錄因子編碼基因CiWRKY2,該基因g DNA為3 224 bp,含有4個(gè)內(nèi)含子;cDNA長2 032 bp,開放閱讀框1 725 bp,可以編碼574個(gè)氨基酸,蛋白分子量大小約為63.29 kD,等電點(diǎn)為7.65。系統(tǒng)進(jìn)化分析發(fā)現(xiàn),CiWRKY2與豆科植物WRKY相似度最高,屬于第一類WRKY蛋白。亞細(xì)胞定位顯示CiWRKY2蛋白定位于細(xì)胞核。實(shí)時(shí)熒光定量結(jié)果表明CiWRKY2主要在葉、根中表達(dá),并且該基因轉(zhuǎn)錄水平的表達(dá)受到干旱、鹽堿、高溫、低溫和ABA誘導(dǎo),表明CiWRKY2基因可能在植物抗逆中起到一定作用。
[Abstract]:A WRKY transcription factor encoding gene CiWRKY2 was cloned from Caragana intermedium by race technique. The gene's g DNA was 3 224 BP, containing 4 introns cDNA length of 2 032 BP, and open reading frame 1 725 BP, which could encode 574 amino acids. The molecular weight of the protein is about 63.29 KD and the isoelectric point is 7.65. Phylogenetic analysis showed that CiWRKY2 had the highest similarity with legume WRKY and belonged to the first kind of WRKY protein. Subcellular localization showed that CiWRKY2 protein was located in the nucleus. The results of real-time fluorescence quantitative analysis showed that CiWRKY2 was mainly expressed in leaves and roots, and the expression of CiWRKY2 was induced by drought, salinization, high temperature, low temperature and ABA, suggesting that CiWRKY2 gene might play a role in plant stress resistance.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(31360169) 內(nèi)蒙古產(chǎn)業(yè)創(chuàng)新創(chuàng)業(yè)人才團(tuán)隊(duì) 內(nèi)蒙古自治區(qū)科技創(chuàng)新團(tuán)隊(duì)(201503004)共同資助
【分類號】：Q943.2

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本文編號：1985168