本文選題：棉花 + BZR基因��； 參考：《棉花學(xué)報(bào)》2017年05期

【摘要】：【目的】油菜素唑抗性因子(Brassinazole resistant transcription factor,BZR)是植物油菜素內(nèi)酯(Brassinosteroid,BR)信號(hào)通路中的1類重要轉(zhuǎn)錄因子,通過調(diào)控相關(guān)基因的表達(dá),參與植物生長(zhǎng)發(fā)育過程。本研究旨在探索棉花中BZR基因的數(shù)量及其功能�！痉椒ā坷蒙镄畔W(xué)方法,對(duì)雷蒙德氏棉、亞洲棉和陸地棉中的BZR基因進(jìn)行全基因組鑒定及表達(dá)模式分析�！窘Y(jié)果】雷蒙德氏棉、亞洲棉和陸地棉中分別有7、7和14個(gè)BZR基因;通過序列特征分析和系統(tǒng)發(fā)育分析可將棉花BZR基因分為2組(a組和b組)。內(nèi)含子/外顯子結(jié)構(gòu)分析發(fā)現(xiàn):大部分BZR基因具有2個(gè)外顯子,a組成員中均具有較長(zhǎng)的內(nèi)含子序列,b組成員的內(nèi)含子序列較短,同組BZR成員具有相似的基因結(jié)構(gòu)�；驈�(fù)制分析表明:片段復(fù)制是棉花BZR基因家族擴(kuò)增的主要方式,棉花BZR基因在進(jìn)化過程中經(jīng)歷了嚴(yán)格的純化選擇。轉(zhuǎn)錄組數(shù)據(jù)分析表明:14個(gè)Gh BZR基因在陸地棉的7個(gè)組織中特異表達(dá),大多數(shù)Gh BZR基因在莖、葉、花瓣和雌蕊中高表達(dá);許多Gh BZR基因的表達(dá)受非生物脅迫誘導(dǎo)�！窘Y(jié)論】推測(cè)Gh BZR基因可能參與棉花抗逆及纖維發(fā)育。本研究結(jié)果可為棉花BZR基因的生物學(xué)功能研究提供信息。
[Abstract]:[objective] Brassinazole resistant transcription factor (BZR) is an important transcriptional factor in the signal pathway of Brassinosteroido Brassinosteroidae, which is involved in plant growth and development by regulating the expression of related genes. The purpose of this study was to explore the number and function of BZR gene in cotton. The BZR gene in Asian cotton and upland cotton were identified and expressed in the whole genome. [results] there were 7 BZR genes and 14 BZR genes in Raymond's cotton, Asian cotton and upland cotton, respectively. Through sequence analysis and phylogenetic analysis, the cotton BZR gene can be divided into two groups: group A and group b. The analysis of intron / exon structure showed that most members of BZR gene had longer intron sequence and shorter intron sequence, and similar gene structure of the same BZR member. Gene replication analysis showed that fragment replication was the main way to amplify the BZR gene family in cotton, and the BZR gene in cotton underwent strict purification and selection during the evolution process. Transcriptome analysis showed that 14 Gh BZR genes were specifically expressed in 7 tissues of Upland cotton, and most of them were highly expressed in stems, leaves, petals and pistil. Many expression of Gh BZR gene was induced by abiotic stress. [conclusion] it is suggested that Gh BZR gene may be involved in stress resistance and fiber development of cotton. The results of this study may provide information for the study of the biological function of cotton BZR gene.
【作者單位】： 中國(guó)農(nóng)業(yè)科學(xué)院棉花研究所/棉花生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;河南科技學(xué)院/現(xiàn)代生物育種河南省協(xié)同創(chuàng)新中心;
【基金】：國(guó)家自然科學(xué)基金(31671745) 國(guó)家重點(diǎn)研發(fā)計(jì)劃(2016YFD0101401,2016YFD0100203)
【分類號(hào)】：S562
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本文編號(hào)：1982830