發(fā)布時(shí)間：2018-06-05 15:38

  本文選題：苦蕎麥 + 鹽脅迫��； 參考：《華北農(nóng)學(xué)報(bào)》2017年04期

【摘要】：為深入研究液泡膜Na~+/H~+逆向轉(zhuǎn)運(yùn)蛋白在植物耐鹽中的作用,以耐鹽苦蕎麥品種川蕎1號為材料,利用同源克隆方法得到NHX基因,命名為FtNHX1,并在Gen Bank中注冊,登錄號為KY438929;序列分析表明,該基因開放閱讀框1 662 bp,共編碼553個(gè)氨基酸,預(yù)測蛋白分子量61.24 kDa,等電點(diǎn)5.15。系統(tǒng)進(jìn)化樹分析表明,FtNHX1與擬南芥(AtNHX1)、水稻(OsNHX1)和小麥(TaNHX1)的親緣關(guān)系較近,氨基酸同源率分別為60.22%,58.95%和57.30%;熒光定量PCR分析表明,隨著Na Cl脅迫濃度的增加,FtNHX1基因在苦蕎麥根部、莖基部和葉片的相對表達(dá)量極顯著增加,150 mmol/L NaCl脅迫下增加幅度最大,分別比對照增加了254.10%,311.35%和256.18%;Na Cl脅迫下FtNHX1基因在苦蕎麥根部、莖基部和葉片的平均表達(dá)量分別比對照增加了109.46%,145.67%和155.94%,莖基部和葉片的平均表達(dá)量較高,說明苦蕎麥FtNHX1基因的表達(dá)明顯受鹽脅迫誘導(dǎo)和調(diào)節(jié),FtNHX1基因與苦蕎麥的耐鹽性有密切聯(lián)系。
[Abstract]:In order to study the role of vacuolar membrane Na ~ / H ~ antiporter in salt tolerance of plants, a salt tolerant buckwheat variety Chuanzao 1 was used to obtain NHX gene named FtNHX1 by homology cloning method, and it was registered in GenBank. The accession number was KY438929, and the sequence analysis showed that the open reading frame 1662 BP encoded 553 amino acids, the predicted molecular weight of protein was 61.24 kDaand the isoelectric point was 5.15. Phylogenetic tree analysis showed that FtNHX1 was closely related to Arabidopsis thaliana AtNHX1, rice OsNHX1) and wheat TaNHX1), the amino acid homology was 60.2222% and 57.300.The fluorescence quantitative PCR analysis showed that FtNHX1 gene was located in the root of buckwheat with the increase of NaCl stress concentration. The relative expression of FtNHX1 in stem base and leaf was significantly increased under 150 mmol / L NaCl stress, and the FtNHX1 gene was increased by 254.1010 ~ 311.35% and 256.1818% respectively in the root of Tartary buckwheat under NaCl stress. The average expression of FtNHX1 in stem base and leaf increased by 145.67% and 155.94%, respectively, and the average expression of FtNHX1 gene in stem base and leaf was higher than that in control, which indicated that the expression of FtNHX1 gene in Tartary buckwheat was induced and regulated by salt stress, and there was a close relationship between FtNHX1 gene and salt-tolerance of Tartary buckwheat.
【作者單位】： 青島農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院山東省高校植物生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金項(xiàng)目(31371552) 山東省自然科學(xué)基金項(xiàng)目(ZR2010CL019)
【分類號】：Q943.2;S517

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