本文選題：沙蔥螢葉甲 + 線粒體COI　； 參考：《環(huán)境昆蟲學報》2017年02期

【摘要】：沙蔥螢葉甲Galeruca daurica(Joannis)是一種近年來在內(nèi)蒙古草原上猖獗發(fā)生為害的新害蟲,為明確內(nèi)蒙古沙蔥螢葉甲不同地理種群間的遺傳多樣性及遺傳分化,本研究測定了內(nèi)蒙古8個地理種群的197頭沙蔥螢葉甲的線粒體COI基因417 bp序列,利用Dna SP 5.0、Arlequin 3.11和Network 4.6.1.0等軟件對沙蔥螢葉甲種群間的遺傳多樣性、遺傳分化、分子變異等進行分析。在所分析的197個COI序列中,共有43個變異位點和62個單倍型,其中2個單倍型為各種群共享,每個種群具有3個及以上的獨享單倍型�？偡N群的單倍型多態(tài)度指數(shù)Hd為0.9466,不同地理種群單倍型多態(tài)度Hd在0.8889-0.9600�？�?cè)后w固定系數(shù)Fst為0.0315,基因流Nm為15.37。中性檢驗結果不顯著(Tajima's D=-1.6300,0.10P0.05),說明內(nèi)蒙古沙蔥螢葉甲在較近的歷史時期內(nèi)沒有出現(xiàn)種群擴張現(xiàn)象。分子變異分析(AMOVA)結果表明,沙蔥螢葉甲的遺傳分化主要來自種群內(nèi)部(96.34%),而種群間未發(fā)生明顯的遺傳分化。各種群間遺傳分化程度與地理距離無顯著相關性。不同地理種群的單倍型在中介網(wǎng)絡圖上散布在不同的分布群中,未形成明顯的地理分布格局。結果表明沙蔥螢葉甲不同地理種群遺傳多樣性高,基因交流程度高,遺傳分化不明顯且與地理距離無顯著相關性。
[Abstract]:Galeruca dauricia Joannis is a new pest that has been rampant in Inner Mongolia grassland in recent years. In this study, the mitochondrial COI gene 417bp sequence was determined in 197 leaf beetles from 8 populations in Inner Mongolia. Genetic diversity and genetic differentiation among leaf beetles were studied by using Dna SP 5.0 Arlequin 3.11 and Network 4.6.1.0, respectively. Molecular variation was analyzed. Among the 197 COI sequences analyzed, there were 43 mutation sites and 62 haplotypes, of which 2 haplotypes were shared by various groups, and each population had three or more exclusive haplotypes. The haplotype multiple attitude index (HD) of total population was 0.946 6, and that of haplotype was 0.8889-0.9600 in different geographic populations. The total population fixed coefficient (Fst) was 0.0315, and the gene flow (Nm) was 15.37. The results of neutral test were not significant. The results showed that there was no population expansion phenomenon in the recent historical period. The results of molecular variation analysis (AMOVA) showed that the genetic differentiation was mainly from within the population, but there was no obvious genetic differentiation among the populations. There was no significant correlation between genetic differentiation and geographical distance among different populations. The haplotypes of different geographical populations are scattered in different distribution groups on the intermediary network map, and there is no obvious geographical distribution pattern. The results showed that the genetic diversity of different geographical populations was high, the degree of gene exchange was high, the genetic differentiation was not obvious and there was no significant correlation between genetic diversity and geographical distance.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學草原昆蟲研究中心;克什克騰旗農(nóng)牧業(yè)局;
【基金】：國家自然科學基金項目(31360441)
【分類號】：S812.6

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