發(fā)布時間：2018-05-31 23:43

  本文選題：豬 + 呼吸道��； 參考：《河南農(nóng)業(yè)科學》2017年09期

【摘要】：為了解河南地區(qū)豬呼吸道隱性感染大腸桿菌對β-內(nèi)酰胺類、四環(huán)素類及氟苯尼考的耐藥情況及耐藥基因的分布,采用微量肉湯稀釋法對從河南地區(qū)豬肺臟分離的31株大腸桿菌進行青霉素、頭孢吡肟、慶大霉素、多西環(huán)素、氟苯尼考等16種藥物敏感性檢測及耐藥表型分析,采用PCR對31株受試大腸桿菌進行β-內(nèi)酰胺類、四環(huán)素類、氟苯尼考耐藥基因檢測。藥敏試驗結果表明,受試菌對青霉素、氨芐西林、阿莫西林3種藥物的耐藥率均達到100%,對頭孢噻呋、頭孢噻肟、頭孢吡肟的耐藥率分別為29.03%、32.26%、3.23%,對四環(huán)素、多西環(huán)素的耐藥率分別為83.87%、70.97%,對氟苯尼考的耐藥率為48.39%,但對喹諾酮類藥物(環(huán)丙沙星、恩諾沙星)、氨基糖苷類藥物(慶大霉素、阿米卡星)較為敏感。耐藥基因檢測發(fā)現(xiàn),β-內(nèi)酰胺類耐藥基因TEM、SHV、CTXM-U、CTX-M-1、CTX-M-9的檢出率分別為54.84%、61.29%、25.81%、9.68%、9.68%;四環(huán)素類耐藥基因tet(A)、tet(M)、tet(L)的檢出率分別為87.10%、54.84%、19.35%,tet(C)、tet(O)陽性菌各1株,未檢出tet(B)、tet(K)、tet(W);氟苯尼考耐藥基因floR檢出率為54.84%。2株菌含有7種耐藥基因,19株菌含有4種及以上耐藥基因。可見,多種耐藥基因共同介導大腸桿菌的多重耐藥性已呈流行趨勢。
[Abstract]:In order to understand the resistance of Escherichia coli to 尾 -lactam, tetracycline and florfenicol and the distribution of drug resistance genes in swine recessive respiratory tract infection in Henan area, Sixteen strains of Escherichia coli isolated from pig lungs in Henan province were tested for penicillin, cefepime, gentamicin, doxycycline, florfenicol, and phenotypic analysis. 尾-lactam, tetracycline and florfenicol resistance genes were detected by PCR in 31 strains of Escherichia coli. The results of drug sensitivity test showed that the resistance rates of the tested bacteria to penicillin, amoxicillin and amoxicillin were 100, and the resistance rates to cefuroxime, cefotaxime and cefepime were 29.03 ~ 32.26 and 3.23, respectively. The drug resistance rates of doxycycline and florfenicol were 83.87 and 48.39, respectively, but sensitive to quinolones (ciprofloxacin, enrofloxacin, aminoglycoside (gentamicin, amikacin). The detection rate of 尾 -lactam resistance gene TEMS-SHVM-UCTX-M-1 CTX-M-9 was 54.84 and 61.29, respectively. The positive rate of tetracycline resistance gene tetAtetMtetetOr (Tet) was 1 strain each, and the positive rate of tetracycline resistance gene tetAtetMtetOL was 87.1010%, 54.84% and 19.35%, respectively, and the positive rate of tetracycline resistance gene (tetAtetMtetOL) was 1 in each of the two strains, and the positive rate of 尾 -lactam resistance gene TEMS-SHVM-UCTX-M-1 CTX-9 was 54.35%. The floR detection rate of florfenicol resistance gene was 54.84.2 strains and 19 strains of florfenicol resistance gene contained 4 or more resistant genes. It can be seen that multidrug resistance of Escherichia coli mediated by multiple drug resistance genes has become prevalent.
【作者單位】： 河南農(nóng)業(yè)大學;
【基金】：國家自然科學基金項目(31201965)
【分類號】：S858.28

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本文編號：1961885