發(fā)布時(shí)間：2018-05-30 13:47

  本文選題：心房顫動 + 轉(zhuǎn)基因　； 參考：《臨床心血管病雜志》2017年05期

【摘要】：目的:研究AAV9-SERCA 2a基因轉(zhuǎn)染對急性心房顫動(房顫)動物模型心房肌細(xì)胞上L型鈣通道表達(dá)的影響。方法:采用60只新西蘭大白兔為實(shí)驗(yàn)對象,采用心包注射法轉(zhuǎn)染AAV9-SERCA 2a基因;將實(shí)驗(yàn)對象隨機(jī)分為4組:房顫組、轉(zhuǎn)熒光蛋白房顫組、轉(zhuǎn)SERCA 2a基因房顫組和對照組。轉(zhuǎn)染后建立急性房顫模型,采用膜片鉗及熒光免疫組織化學(xué)法檢測心房肌細(xì)胞上L型鈣通道的電流變化及其L型鈣通道蛋白表達(dá)的情況。結(jié)果:轉(zhuǎn)SERCA 2a基因房顫組心房肌細(xì)胞上LVDCCa1c蛋白表達(dá)明顯高于房顫組(P0.05),與對照組比較差異無統(tǒng)計(jì)學(xué)意義。轉(zhuǎn)SERCA 2a基因房顫組心房肌細(xì)胞上L型鈣通道電流密度高于房顫組,差異有統(tǒng)計(jì)學(xué)意義(P0.05),與對照組比較差異無統(tǒng)計(jì)學(xué)意義。結(jié)論:轉(zhuǎn)SERCA 2a基因治療可減少急性房顫模型心房肌細(xì)胞鈣超載,減少房顫對L型鈣通道電流密度的影響,對防止心臟電重構(gòu)具有積極的意義。
[Abstract]:Aim: to study the effect of AAV9-SERCA 2a gene transfection on the expression of L-type calcium channel in atrial myocytes of acute atrial fibrillation (AF) animal model. Methods: sixty New Zealand white rabbits were used to transfect AAV9-SERCA 2a gene by pericardial injection. The subjects were randomly divided into 4 groups: atrial fibrillation group, fluorescent protein atrial fibrillation group, SERCA 2a gene atrial fibrillation group and control group. The acute atrial fibrillation model was established after transfection. The changes of L-type calcium channel current and the expression of L-type calcium channel protein in atrial myocytes were detected by patch clamp and fluorescence immunohistochemistry. Results: the expression of LVDCCa1c protein in atrial myocytes in SERCA 2a gene group was significantly higher than that in atrial fibrillation group (P 0.05). The L-type calcium channel current density in atrial myocytes in SERCA 2a gene group was significantly higher than that in atrial fibrillation group (P 0.05), but there was no significant difference compared with the control group. Conclusion: SERCA 2a gene therapy can reduce the calcium overload of atrial myocytes in acute atrial fibrillation model, reduce the effect of atrial fibrillation on L-type calcium channel current density, and have a positive significance in preventing cardiac electrical remodeling.
【作者單位】： 新疆醫(yī)科大學(xué)第一附屬醫(yī)院內(nèi)科;新疆醫(yī)科大學(xué)第一附屬醫(yī)院干部綜合內(nèi)科;
【分類號】：R-332;R541.75

【相似文獻(xiàn)】

相關(guān)期刊論文 前6條

1 陳伯成,胡志安,羅峻;海馬長時(shí)程增強(qiáng)中一氧化氮變化與L型鈣通道和神經(jīng)細(xì)胞黏附分子的關(guān)系[J];中國臨床康復(fù);2004年16期

2 宋立林,張翼,谷雙振,盧慎圭,周兆年;雌二醇對豚鼠心室肌細(xì)胞L型鈣通道的抑制作用[J];中國應(yīng)用生理學(xué)雜志;2000年02期

3 高瞻,朱妙章,周京軍,王躍民,梁濤,張美芳;酸中毒對大鼠心室肌細(xì)胞L型鈣通道的影響[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2001年21期

4 李世英,歐陽守;豚鼠結(jié)腸平滑肌細(xì)胞L型鈣通道特性研究[J];基礎(chǔ)醫(yī)學(xué)與臨床;2003年S1期

5 王旭良;鐘衛(wèi)民;張陽;裴兆輝;張燕;陳柯;;次聲作用后大鼠心肌細(xì)胞內(nèi)鈣離子變化與L型鈣通道、Ca~(2+)-ATP酶及Na~+-K~+-ATP酶變化的關(guān)系[J];中國組織工程研究與臨床康復(fù);2007年31期

6 ;[J];;年期

相關(guān)會議論文 前2條

1 馬勇;徐又佳;;鐵代謝的調(diào)節(jié)肽鐵調(diào)素通過L型鈣通道影響成骨細(xì)胞鈣離子轉(zhuǎn)運(yùn)和細(xì)胞礦化[A];中華醫(yī)學(xué)會第六次全國骨質(zhì)疏松和骨礦鹽疾病學(xué)術(shù)會議暨中華醫(yī)學(xué)會骨質(zhì)疏松和骨礦鹽疾病分會成立十周年論文匯編[C];2011年

2 丁軍;許彤輝;施玉j;;川楝素通過L型鈣通道提高NG108-15細(xì)胞內(nèi)游離鈣濃度[A];中國毒理學(xué)會生物毒素毒理專業(yè)委員會第4次、中國生物化學(xué)與分子生物學(xué)會毒素專業(yè)組第5次學(xué)術(shù)研討會論文摘要[C];2001年

，

本文編號：1955515