發(fā)布時(shí)間：2018-05-29 02:00

  本文選題：星油藤 + α-亞麻油酸��； 參考：《植物生理學(xué)報(bào)》2017年01期

【摘要】：α-亞麻油酸(α-linolenic acid,ALA)對防治脂類代謝失調(diào)而引起的肥胖、心血管疾病、高血脂、高血壓等慢性疾病具有重要的生理功能。然而,大部分油料作物種子ALA的含量都很低,提高種子ALA的含量一直是油料作物品質(zhì)改良的重點(diǎn)內(nèi)容之一。星油藤(Plukenetia volubilis)種子油中富含ALA,是一種具有重要開發(fā)前景的油料植物,解析其種子高效累積ALA的分子機(jī)理不僅能為星油藤的遺傳改良提供理論依據(jù),而且能夠?yàn)樘岣哂土献魑锓N子ALA含量的遺傳育種提供理論參考。前期的研究發(fā)現(xiàn)星油藤種子高效累積ALA主要是由于關(guān)鍵酶基因Pv FAD3高度表達(dá)的結(jié)果。為了進(jìn)一步理解星油藤種子在發(fā)育過程中Pv FAD3的被調(diào)控機(jī)理,本研究利用染色體步移法克隆了Pv FAD3的啟動子,并且利用生物信息學(xué)分析、鑒別了啟動子潛在的關(guān)鍵調(diào)控元件,并重點(diǎn)圍繞可能調(diào)控Pv FAD3基因表達(dá)的b ZIP124轉(zhuǎn)錄因子,通過酵母單雜交和煙草葉片啟動子激活實(shí)驗(yàn),驗(yàn)證了Pv FAD3的表達(dá)受b ZIP124轉(zhuǎn)錄因子的調(diào)控。該研究為理解植物種子高效累積ALA的分子機(jī)理提供了新的證據(jù)。
[Abstract]:偽-linolenic acid (偽-linolenic acid ALAs) plays an important physiological role in the prevention and treatment of obesity, cardiovascular disease, hyperlipidemia, hypertension and other chronic diseases caused by dyslipidemia. However, the content of ALA in seed of most oil crops is very low, and increasing the content of seed ALA is always one of the key contents of oil crop quality improvement. The seed oil of Plukenetia volubilis (Plukenetia volubilis) is rich in ALAs and is a promising oil plant. The molecular mechanism of ALA accumulation in its seeds can not only provide a theoretical basis for genetic improvement. Moreover, it can provide theoretical reference for genetic breeding of improving ALA content of oil crop seeds. In previous studies, it was found that the high accumulation of ALA in seeds was mainly due to the high expression of the key enzyme gene PV FAD3. In order to further understand the regulated mechanism of PV FAD3 during development, the promoter of PV FAD3 was cloned by chromosome step method, and the potential key regulatory elements of the promoter were identified by bioinformatics analysis. The b ZIP124 transcriptional factors which may regulate the expression of PV FAD3 gene were focused on. The results showed that the expression of PV FAD3 was regulated by b ZIP124 transcription factors through yeast single hybridization and tobacco leaf promoter activation experiments. This study provides new evidence for understanding the molecular mechanism of ALA accumulation in plant seeds.
【作者單位】： 中國科學(xué)院昆明植物研究所;中國科學(xué)院大學(xué);周口師范學(xué)院生命科學(xué)系;
【基金】：國家自然科學(xué)基金(31571709) 云南省應(yīng)用基礎(chǔ)自然科學(xué)基金(2014FB172)~~
【分類號】：Q943.2;S565.9
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本文編號：1949062