發(fā)布時間：2018-05-28 23:48

  本文選題：菜薹 + 蛋白甲基轉(zhuǎn)移酶�。� 參考：《園藝學(xué)報》2017年06期

【摘要】：以‘油青49’和‘油青甜菜薹80’菜薹莖尖為材料,采用RT-PCR和RACE技術(shù)克隆獲得組蛋白甲基轉(zhuǎn)移酶基因Brcu PRMT5的全長cDNA和gDNA序列。BrcuPRMT5 cDNA序列全長為2 117 bp,其中完整開放閱讀框為1 929 bp,編碼642個氨基酸,相對分子量為71.55 kD,理論等電點(p I)為5.87;多序列比對結(jié)果表明,Brcu PRMT5編碼的氨基酸序列含有高等植物PRMT5基因1個高度保守的結(jié)構(gòu)域;系統(tǒng)發(fā)育分析結(jié)果顯示與大白菜、油菜及甘藍(lán)的親緣關(guān)系最近;亞細(xì)胞定位軟件分析得知,Brcu PRMT5蛋白無跨膜區(qū)域,可能定位于線粒體中;對應(yīng)的gDNA全長為4 151 bp,含有23個外顯子和22個內(nèi)含子,最長的外顯子長度為140 bp,內(nèi)含子的長度范圍為50~150 bp。利用半定量RT-PCR和實時熒光定量PCR技術(shù)分析基因的表達(dá),BrcuPRMT5在菜薹不同組織中均有表達(dá),其中在花中表達(dá)量最高,葉次之,根中最低;BrcuPRMT5從苗期至完全抽薹開花期的表達(dá)量呈現(xiàn)上升趨勢。BrcuPRMT5在菜薹花發(fā)育過程中可能起一定的調(diào)控作用。
[Abstract]:The stem tips of 'Youqing 49' and 'you Qing sweet' cabbage bolting 80 'were used as materials. The full length cDNA and gDNA sequence. BrcuPRMT5 cDNA sequence of histone methyltransferase gene Brcu PRMT5 were cloned by RT-PCR and RACE techniques. The complete open reading frame was 1 929 BP, encoding 642 amino acids. The relative molecular weight was 71.55 KD and the theoretical isoelectric point I) was 5.87. The results of multiple sequence alignment showed that the amino acid sequence encoded by Brcu PRMT5 contained a highly conserved domain of PRMT5 gene in higher plants, and phylogenetic analysis showed that the amino acid sequence of Brcu PRMT5 was similar to that of Chinese cabbage. The relationship between Brcu and Brcu in Brassica napus was very close. The subcellular localization software showed that the Brcu PRMT5 protein was located in mitochondria and had no transmembrane region. The corresponding gDNA was 4 151 BP with 23 exons and 22 introns. The longest exon length is 140 BP, and the intron length range is 50 ~ 150 BP. The expression of BrcuPRMT5 was detected by semi-quantitative RT-PCR and real-time fluorescence quantitative PCR in different tissues of Chinese cabbage, in which the highest expression was found in flower, followed by leaf. The expression of BrcuPRMT5 increased from seedling stage to full bolting flowering stage. BrcuPRMT5 may play a regulatory role in the development of cauliflower.
【作者單位】： 江西農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;仲愷農(nóng)業(yè)工程學(xué)院;
【基金】：國家自然科學(xué)基金項目(31360484) 教育部高等學(xué)校博士學(xué)科點專項科研基金項目(20133603120006) 江西省自然科學(xué)基金項目(2013BAB204009) 江西農(nóng)業(yè)大學(xué)博士啟動基金項目(3181)
【分類號】：S634.5
，

本文編號：1948667