發(fā)布時間：2018-05-28 02:27

  本文選題：攀鱸 + 遺傳多樣性�。� 參考：《南方水產(chǎn)科學》2017年03期

【摘要】：測定了中國華南6水系及瀾滄江(云南勐臘)-湄公河流域(柬埔寨洞里薩湖)的125尾攀鱸(Anabas testudineus)線粒體部分ND2基因1 010 bp序列,分析發(fā)現(xiàn)39個變異位點和12個單倍型,總遺傳多樣性較低(h=0.369,π=0.003 8),推測可能經(jīng)歷過嚴重的瓶頸效應(yīng);中國攀鱸群體遺傳多樣性更低(h=0.282,π=0.000 4),處于邊緣區(qū)的中國攀鱸群體是造成低遺傳多樣性的主要原因。在單倍型網(wǎng)絡(luò)圖中柬埔寨和中國攀鱸各自聚類,具有明顯地理結(jié)構(gòu)和譜系結(jié)構(gòu),推測地質(zhì)運動和氣候變化導致基因交流受阻所致。核苷酸錯配圖和中性檢驗表明中國群體經(jīng)歷過種群擴張,時間約為(5.94~4.13)萬年前。華南水系群體間基因交流通暢,不存在明顯分化;但與云南瀾滄江群體間分化大而顯著(FST=0.775,P0.01),AMOVA分析顯示變異主要來自組群間(77.41%),推測二者分化時間約為(4.0~2.8)萬年前,云南群體受末次冰期的影響,基因交流受阻而出現(xiàn)分化。中國群體和柬埔寨群體可作為2個管理單位進行保護;就中國群體而言,韓江水系群體遺傳多樣性最高,建議優(yōu)先保護;瀾滄江與華南水系間群體分化顯著且遺傳多樣性極低,建議對瀾滄江水系群體進行保護,以避免種質(zhì)資源滅絕。
[Abstract]:The mitochondrial ND2 gene of Anabas Testudineus in 6 drainage systems of South China and Lancang River (Lake Donglisa, Cambodia) was determined. 39 mutation sites and 12 haplotypes were found. The total genetic diversity was 0.369, 蟺 ~ 0.003 ~ 8 ~ 8, which may have experienced severe bottleneck effect, and the genetic diversity of Chinese climbing perch population was lower than 0.282, 蟺 ~ (0.000) 4m, and the main reason of low genetic diversity was the Chinese climbing perch population in the marginal area. In the haplotype network map, Cambodian and Chinese climbing perch have distinct geographical structure and pedigree structure respectively. It is speculated that geological movement and climate change lead to blocked gene exchange. The nucleotide mismatch map and neutral test showed that the population in China had experienced population expansion with a time of about 5.94 to 4.13 million years ago. There was no obvious differentiation between the populations of southern China and Lancang River, but the AMOVA analysis showed that the variation was mainly from 77.41% of different groups, and the time of differentiation between the two populations was about 4.0 ~ 2.8) ten thousand years ago, there was a significant difference between the populations of Lancang River and Yunnan Province, but the AMOVA analysis showed that the difference between the two populations was significant, and the variation was mainly due to the variation of 77.41%, which was estimated to be about 4.0% 2. 8 years ago. Yunnan population was affected by the last glacial period and gene exchange was blocked and differentiated. The Chinese population and the Cambodian population can be protected as two management units; for the Chinese population, the Han River population has the highest genetic diversity and priority should be given to protection; the population differentiation between Lancang River and South China is significant and the genetic diversity is very low. In order to avoid extinction of germplasm resources, the population of Lancang River system should be protected.
【作者單位】： 暨南大學水生生物研究中心熱帶亞熱帶水生態(tài)工程教育部工程研究中心;廣州市水務(wù)科學研究所;
【基金】：國家自然科學基金項目(41071034) 中央高�；究蒲袠I(yè)務(wù)費專項資金項目(21613105) 廣州市科技計劃項目(201508020104)
【分類號】：S917.4

【相似文獻】

相關(guān)期刊論文 前5條

1 梁松,楊大偉,馬海欨;攀鱸魚養(yǎng)殖技術(shù)[J];中國水產(chǎn);2002年09期

2 梁松,楊大偉 ,杜永寧;攀鱸池塘高產(chǎn)養(yǎng)殖試驗[J];科學養(yǎng)魚;2002年03期

3 陳祈輝,陳一通,王好鎮(zhèn);攀鱸人工繁殖及育苗技術(shù)初探[J];福建水產(chǎn);2003年02期

4 梁松;攀鱸的人工繁殖與種苗培育[J];科學養(yǎng)魚;2004年11期

5 ;[J];;年期

，

本文編號：1944861