發(fā)布時間：2018-05-27 22:23

  本文選題：結(jié)腸癌 + 慢病毒��； 參考：《腫瘤防治研究》2017年01期

【摘要】：目的探討GTPBP4基因沉默后,RKO細胞增殖和凋亡等生物學行為的改變。方法將慢病毒GTPBP4-siRNA及CON053陰性病毒轉(zhuǎn)染結(jié)腸癌RKO細胞株,以Real-time PCR和Western blot檢測敲減效率。Cellomics細胞計數(shù)檢測細胞生長,MTT法檢測細胞增殖,FACS法進行細胞周期檢測,并進行細胞克隆形成實驗,AnnexinⅤ-APC單染法流式細胞儀檢測細胞凋亡。結(jié)果慢病毒成功感染RKO細胞,m RNA和蛋白檢測均顯示GTPBP4基因敲減成功。GTPBP4基因敲減后,RKO細胞增殖速率受到顯著抑制,MTT值比值(即增殖倍數(shù))減小,G_(0/1)、G_2/M期細胞顯著增多,S期明顯減少,細胞克隆集落數(shù)目減少,凋亡峰值明顯高于對照組,且峰值出現(xiàn)時間早于對照組。結(jié)論 GTPBP4基因可能通過促進腫瘤細胞增殖、抑制凋亡而影響結(jié)腸癌的發(fā)生發(fā)展。
[Abstract]:Objective to investigate the changes of proliferation and apoptosis of GTPBP4 cells after GTPBP4 gene silencing. Methods Lentivirus GTPBP4-siRNA and CON053 negative viruses were transfected into colon cancer RKO cell lines. Cell proliferation was detected by Real-time PCR and Western blot assay. Cell proliferation was detected by cell growth assay. Cell apoptosis was detected by Annexin 鈪，

本文編號：1944044