本文選題：糖尿病胃輕癱 + 大鼠��； 參考：《浙江大學(xué)》2017年碩士論文

【摘要】：目的:觀察"健脾理氣合劑"對糖尿病胃輕癱(diabetic gastroparesis,DGP)大鼠胃動(dòng)力指標(biāo)、胃組織細(xì)胞凋亡及相關(guān)基因bcl-2/bax表達(dá)的影響,探討"健脾理氣合劑"治療DGP的效應(yīng)與機(jī)理。方法:采用Wistar大鼠通過腹腔注射2%鏈脲佐菌素(STZ)溶液造模糖尿病大鼠,通過灌服熟地水煎液誘導(dǎo)DGP大鼠模型。將造模的大鼠隨機(jī)分為模型組,健脾理氣合劑高、低劑量組以及嗎丁啉組。各組連續(xù)治療或正常飼養(yǎng)3周。治療結(jié)束后檢測各項(xiàng)指標(biāo):包括大鼠體重、血糖、血漿胃動(dòng)素濃度、胃排空率、胃平滑肌細(xì)胞凋亡以及胃組織的bcl-2/bax基因表達(dá)。結(jié)果:DGP大鼠體重較正常大鼠體重明顯降低(P0.001),經(jīng)健脾理氣合劑及嗎丁啉溶液灌胃治療3周后,體重較模型組增加(P0.05)。DGP大鼠在各個(gè)監(jiān)測點(diǎn)的平均血糖濃度均較正常組顯著升高(P0.001),健脾理氣合劑高、低劑量組平均血糖濃度均明顯低于模型組(P0.05),健脾理氣合劑高劑量組血糖濃度高于其他DGP大鼠(P0.05)。模型組大鼠的胃內(nèi)色素殘留率和血漿胃動(dòng)素濃度均明顯高于正常組和健脾理氣合劑高劑量組(P0.05);健脾理氣合劑高劑量組大鼠的胃內(nèi)色素殘留率和血漿胃動(dòng)素濃度均低于嗎丁啉組和低劑量組(P0.05),與正常組相比無顯著差異(P0.05)。DGP大鼠胃壁組織中的平滑肌細(xì)胞均有不同程度的肌細(xì)胞排列紊亂及空泡樣變,以模型組與嗎丁啉組最明顯,健脾理氣合劑低劑量組次之,健脾理氣合劑高劑量組最輕。DGP大鼠胃平滑肌細(xì)胞凋亡率跟正常組相比均有升高(P0.001),其中模型組明顯高于健脾理氣合劑高、低劑量組(P0.05),但與嗎丁啉組相比無明顯差異(P0.05),健脾理氣合劑高劑量組的凋亡率高于其他造模各組(P0.05)。DGP大鼠胃平滑肌細(xì)胞的bcl-2/bax蛋白表達(dá)存在明顯異常,而且兩種基因蛋白的表達(dá)呈現(xiàn)負(fù)相關(guān)。模型組的bcl-2蛋白表達(dá)明顯低于正常組、健脾理氣合劑高、低劑量組(P0.001),但與嗎丁啉組無明顯差異(P0.05);健脾理氣合劑高劑量組高于其他造模各組(P0.05),但低于正常組(P0.05);bax蛋白表達(dá)檢測值各組比較的結(jié)果與bcl-2相同,但數(shù)值上表現(xiàn)為負(fù)相關(guān),即模型組大鼠胃平滑肌細(xì)胞中bax蛋白表達(dá)明顯高于正常組、健脾理氣合劑高、低劑量組(P0.001),但與嗎丁啉組無明顯差異(P0.05),健脾理氣高劑量組明顯低于其他造模各組(P0.05),但低于正常組(P0.05)。結(jié)論:DGP大鼠與正常大鼠相比,體重明顯減輕,血糖明顯增高,存在明顯的胃動(dòng)力障礙。健脾理氣合劑高劑量灌胃可明顯增加DGP大鼠的體重、降低血糖,減少胃內(nèi)色素殘留率,促進(jìn)血漿胃動(dòng)素分泌,改善胃壁平滑肌細(xì)胞的病理變化,對糖尿病胃輕癱具有一定的治療作用。DGP大鼠胃壁平滑肌細(xì)胞存在bax和bcl-2基因蛋白的異常表達(dá),兩者呈現(xiàn)負(fù)相關(guān)。健脾理氣合劑可以升高胃壁平滑肌細(xì)胞的bcl-2蛋白表達(dá)、降低bax蛋白表達(dá),對bcl-2/bax的蛋白表達(dá)的良性調(diào)控有可能成為其治療糖尿病胃輕癱的作用機(jī)制之一。
[Abstract]:Objective: to observe the effect of "Jianpi Liqi mixture" on gastric dynamic index, gastric tissue apoptosis and the expression of related gene bcl-2/bax in diabetic gastroparesis rats, and to explore the effect and mechanism of "Jianpi Liqi mixture" in treating DGP. Methods: diabetic rats were induced by intraperitoneal injection of 2% streptozotocin (STZ) solution in Wistar rats, and DGP rats were induced by oral administration of cooked water decoction. The rats were randomly divided into model group, Jianpi Liqi mixture group, low dose group and morpholine group. Each group was treated continuously or fed normally for 3 weeks. At the end of the treatment, the indexes included body weight, blood glucose, plasma motilin concentration, gastric emptying rate, apoptosis of gastric smooth muscle cells and bcl-2/bax gene expression in gastric tissue. Results the body weight of DGP rats was significantly lower than that of normal rats. The rats were treated with Jianpi Liqi mixture and morpholine solution for 3 weeks. Compared with the model group, the average blood glucose concentration in the model group was significantly higher than that in the normal group, and the concentration of blood glucose in the DGP rats was significantly higher than that in the normal group, and the mixture of invigorating spleen and lipophilic qi was higher than that in the normal group. The average blood glucose concentration in the low dose group was significantly lower than that in the model group, and that in the high dose group of Jianpi Liqi mixture was higher than that in other DGP rats. The residual rate of gastric pigments and plasma motilin concentration in the model group were significantly higher than those in the normal group and the high dose group of Jianpi Liqi mixture (P 0.05), while the residual rate of the gastric pigment and the plasma motilin concentration in the high dose group of Jianpi Li Qi mixture were both higher than those in the normal group and the high dose group of Jianpi Li Qi mixture. Compared with the normal group, the smooth muscle cells in the gastric wall tissue of the DGP rats had different degrees of myocyte arrangement disorder and vacuolar degeneration, but there was no significant difference between the morpholine group and the low dose group (P 0.05), and there was no significant difference between the control group and the normal group. The model group and morpholine group were the most obvious, followed by the low dose group of Jianpi Liqi mixture. The apoptosis rate of gastric smooth muscle cells in the high dose group of Jianpi Liqi mixture was significantly higher than that in the normal group, especially in the model group, which was significantly higher than that in the model group, and the apoptosis rate of gastric smooth muscle cells in the DGP group was higher than that in the normal group. The apoptosis rate of high dose group of Jianpi Liqi mixture was significantly higher than that of other groups. The expression of bcl-2/bax protein in gastric smooth muscle cells of rats in low dose group was higher than that in other groups, but there was no significant difference compared with that in morpholine group, and the expression of bcl-2/bax protein in gastric smooth muscle cells of rats with high dose of Jianpi Liqi mixture was obviously abnormal. Moreover, the expression of the two gene proteins was negatively correlated. The expression of bcl-2 protein in the model group was significantly lower than that in the normal group. In the low dose group, there was no significant difference with the morpholine group (P 0.05), the high dose group of Jianpi Liqi mixture was higher than that of the other model making groups (P 0.05), but the result was the same as that of the normal group (P 0.05), but the value was negatively correlated with that of the normal group. The expression of bax protein in the gastric smooth muscle cells in the model group was significantly higher than that in the normal group. The expression of bax protein in the model group was significantly higher than that in the control group. The expression of bax protein in the gastric smooth muscle cells of the model group was higher than that in the normal group. Conclusion compared with the normal rats, the weight and blood glucose of the DGP rats were significantly decreased, and there were obvious gastric motility disorders. High dose of Jianpi Liqi mixture could significantly increase the weight of DGP rats, decrease blood sugar, reduce the residual rate of gastric pigment, promote the secretion of motilin in plasma, and improve the pathological changes of smooth muscle cells in gastric wall. There were abnormal expressions of bax and bcl-2 gene protein in gastric wall smooth muscle cells of diabetic gastroparesis rats. Jianpi Liqi mixture can increase the expression of bcl-2 protein and decrease the expression of bax protein in gastric wall smooth muscle cells. The benign regulation of bcl-2/bax protein expression may be one of the mechanisms of its treatment of diabetic gastroparesis.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R285.5

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