本文選題：野生蕉 + 密碼子偏性��； 參考：《福建農(nóng)業(yè)學(xué)報(bào)》2017年05期

【摘要】：為了解FAD7的密碼子使用特性,試驗(yàn)采用CodonW、SPSS19.0、MEGA5.0等軟件和EMBOSS在線程序?qū)σ吧禙AD7密碼子偏性進(jìn)行分析,同時(shí)與其他單/雙子葉植物FAD7和模式生物基因組進(jìn)行比較。結(jié)果顯示,野生蕉FAD7密碼子偏性水平較弱,密碼子組成和結(jié)尾偏好使用G或C,而CUC、CAG和AGG等在該基因中具有較高的使用頻率;物種間FAD7比較結(jié)果發(fā)現(xiàn),單子葉植物FAD7密碼子組成明顯偏好G或C,而雙子葉植物則完全相反;基于密碼子使用頻率FAD7聚類結(jié)果與CDS分類結(jié)果一致,均能將單/雙子葉植物區(qū)分開(kāi)來(lái);此外,大腸桿菌原核表達(dá)受體系統(tǒng)可作為野生蕉FAD7基因異源表達(dá)理想試驗(yàn)體系。研究結(jié)果為野生蕉FAD7后續(xù)結(jié)構(gòu)和功能研究提供了科學(xué)依據(jù)。
[Abstract]:In order to understand the codon usage characteristics of FAD7, the codon, SPSS 19.0, MEGA 5.0 and EMBOSS online program were used to analyze the codon bias of wild banana FAD7, and the codon bias was compared with that of other monocotyledonous plants FAD7 and model organism genomes. The results showed that the codon bias of wild banana FAD7 was weak, and the codon composition and ending preferred to use G or C, while Cuccinia CAG and AGG had a higher frequency of use in the gene, and the results of FAD7 comparison among species showed that, Monocotyledon FAD7 codon composition obviously preferred G or C, while dicotyledonous plants were completely opposite. The results of codon frequency FAD7 clustering were consistent with CDS classification results, which could distinguish monocotyledonous plants from dicotyledonous plants. E. coli prokaryotic expression receptor system can be used as an ideal test system for heterologous expression of FAD7 gene in wild plantain. The results provide a scientific basis for the study of the structure and function of wild banana FAD7.
【作者單位】： 電子科技大學(xué)計(jì)算機(jī)科學(xué)與工程學(xué)院;福建農(nóng)林大學(xué)園藝植物生物工程研究所;
【基金】：福建省科技重大專項(xiàng)(2015NZ0002) 國(guó)家香蕉產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)(CARS-32-11)
【分類號(hào)】：S668.1

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