本文選題：山羊 + KLF　； 參考：《農(nóng)業(yè)生物技術(shù)學(xué)報》2017年01期

【摘要】：Krüppel樣因子(Krüppel-like factors,KLFs)是一組與真核基因轉(zhuǎn)錄調(diào)控密切相關(guān)的鋅指蛋白。本研究旨在克隆山羊(Capra hircus)KLF13基因的CDS區(qū)序列,檢測其在山羊不同組織中表達水平,并鑒定在細胞分化過程中的表達規(guī)律。隨機選取24月齡的健康簡州大耳羊公羊4只,屠宰后分別采集心、肝、脾、肺、腎、皮下脂肪、背最長肌、股二頭肌和臂三頭肌組織樣品,提取組織總RNA,利用RT-PCR法克隆山羊KLF13基因序列,并進行生物信息學(xué)分析;利用實時定量PCR法檢測KLF13 m RNA的表達水平,并檢測前體脂肪細胞(0、3、5、7 d)和成肌細胞(0、2、3、4、5 d)誘導(dǎo)分化后的KLF13 m RNA表達水平。結(jié)果顯示,克隆得到KLF13 c DNA序列長991 bp(Gen Bank登陸號:KU041755),其中包含CDS區(qū)876 bp,5'UTR序列12 bp,3'UTR序列103 bp,編碼292個氨基酸殘基。山羊KLF13基因編碼的氨基酸序列與綿羊(Ovis aries)、牛(Bos taurus)、豬(Sus scrofa)、小鼠(Mus musculus)的相似性分別為100%、97.9%、96.6%和90.8%。山羊KLF13基因與綿羊具有最近的親緣關(guān)系,其次為牛、人(Homo sapiens)、豬和小鼠。KLF13 m RNA在山羊脾和肺中具有最高的表達水平,而在肌肉中的表達豐度較低。在前體脂肪細胞的分化過程中KLF13m RNA表達水平逐漸降低,而在成肌細胞分化過程中則逐漸上升(除5 d外)。結(jié)果提示,KLF13基因可能在調(diào)控山羊前體脂肪細胞分化過程中具有重要作用。本實驗為研究KLF13基因在山羊前體脂肪細胞分化中的作用提供了基礎(chǔ)資料。
[Abstract]:Kr 眉 ppel like factor Kr 眉 ppel-like factors KLFs is a group of zinc finger proteins closely related to the transcriptional regulation of eukaryotic genes. The purpose of this study was to clone the CDS sequence of Capra hircus)KLF13 gene from goat, detect its expression level in different tissues of goat, and identify the expression pattern of capra hircus)KLF13 gene in the process of cell differentiation. Samples of heart, liver, spleen, lung, kidney, subcutaneous fat, longissimus dorsi muscle, biceps femoris muscle and triceps arm muscle were collected after slaughter. The total RNAs were extracted, the goat KLF13 gene sequence was cloned by RT-PCR method, and bioinformatics analysis was carried out. The expression level of KLF13 m RNA was detected by real-time quantitative PCR. The expression of KLF13 m RNA in preadipocytes and myoblasts were detected after induction of differentiation for 5 d. The results showed that the length of the KLF13 c DNA sequence was 991 bp(Gen Bank, which contained the CDS region 876 BP ~ (5) ~ (5) UTR sequence (12 BP ~ (3) UTR sequence (103 BP), encoding 292 amino acid residues. The amino acid sequence of goat KLF13 gene was similar to that of Ovis ariesus, Bos taurus, Sus scrofaus and Mus musculus, respectively. The similarity of amino acid sequence of goat KLF13 gene to Ovis ariesus, Bos taurus, Sus scrofaus and Mus musculus was 96. 6% and 98. 8%, respectively. Goat KLF13 gene was closely related to sheep, followed by cattle, Homo sapiensus, pig and mouse. KLF13m RNA had the highest expression level in goat spleen and lung, but lower expression in muscle. During the differentiation of preadipocytes, the expression of KLF13m RNA decreased gradually, but increased gradually during the differentiation of myoblasts (except for 5 days). These results suggest that KLF13 gene may play an important role in regulating the differentiation of goat precursor adipocytes. This study provides basic data for the study of the role of KLF13 gene in the differentiation of goat precursor adipocytes.
【作者單位】： 青藏高原動物遺傳資源保護與利用四川省重點實驗室;西南民族大學(xué)生命科學(xué)與技術(shù)學(xué)院;四川省畜牧科學(xué)研究院;
【基金】：中央高�；究蒲袠I(yè)務(wù)費專項資金項目(No.2016NZYQN36)
【分類號】：S827
，

本文編號：1916240