本文選題：骨關(guān)節(jié)炎 + 差異表達(dá)基因��； 參考：《中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志》2017年09期

【摘要】：目的:探討前交叉韌帶(anterior cruciate ligament,ACL)離斷聯(lián)合半月板切除誘發(fā)的骨關(guān)節(jié)炎(osteoarthritis,OA)的基因表達(dá)和生物過(guò)程的改變,為OA分子機(jī)制的進(jìn)一步研究提供生物信息學(xué)依據(jù)。方法:從GEO數(shù)據(jù)庫(kù)下載Appleton等構(gòu)建的ACL離斷聯(lián)合半月板切除誘發(fā)的OA早期大鼠模型的基因芯片數(shù)據(jù)集,應(yīng)用生物信息學(xué)方法篩選差異表達(dá)基因(differentially expressed genes,DEGs),并進(jìn)行基因本體論(Gene ontology,GO)和日本京都基因和基因組百科全書(shū)代謝通路數(shù)據(jù)庫(kù)(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。結(jié)果:共篩選出170個(gè)DEGs,其中包括97個(gè)上調(diào)基因和73個(gè)下調(diào)基因。上調(diào)基因主要富集在細(xì)胞外基質(zhì),并與細(xì)胞外基質(zhì)交互通路等信號(hào)通路關(guān)系密切。而下調(diào)基因主要富集在肌肉收縮的生物學(xué)功能中,并與PPAR通路等信號(hào)通路關(guān)系密切。結(jié)論:細(xì)胞外基質(zhì)和肌肉收縮的改變對(duì)OA的發(fā)生發(fā)展起著關(guān)鍵作用。細(xì)胞外基質(zhì)受體交互通路和PPAR信號(hào)通路與OA密切相關(guān),值得進(jìn)一步深入研究。
[Abstract]:Objective: to investigate the gene expression and biological process of osteoarthritis induced by anterior cruciate ligament amputation combined with meniscectomy, and to provide bioinformatics basis for further study on the molecular mechanism of OA. Methods: the gene-chip data set of the early OA rat model induced by ACL disconnection and meniscectomy was obtained by downloading Appleton from GEO database. Bioinformatics method was used to screen differentially expressed genes (expressed genesus) and to analyze the enrichment of gene ontology (GOG) and Kyoto Encyclopedia of Genes and genomes (KEGG) pathway in Kyoto gene and genome encyclopedia of Japan. Results: 170 DEGs were screened, including 97 up-regulated genes and 73 down-regulated genes. The up-regulated genes are mainly concentrated in extracellular matrix and are closely related to signal pathways such as extracellular matrix interaction pathway. The down-regulated genes are mainly concentrated in the biological function of muscle contraction and are closely related to signal pathways such as PPAR pathway. Conclusion: the changes of extracellular matrix and muscle contraction play a key role in the development of OA. Extracellular matrix receptor interaction pathway and PPAR signaling pathway are closely related to OA.
【作者單位】： 復(fù)旦大學(xué)附屬華山醫(yī)院運(yùn)動(dòng)醫(yī)學(xué)科;
【基金】：國(guó)家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)(2015AA033703) 國(guó)家自然科學(xué)基金(81572108,81370052) 國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFC1100300)
【分類(lèi)號(hào)】：R684.3

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