本文選題：十字花科黑腐病菌 + rpf/DSF系統(tǒng)��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年08期

【摘要】：十字花科黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)的Rpf C/Rpf G雙組分系統(tǒng)感應(yīng)DSF(diffusible signal factor)因子,并通過二級信使環(huán)二鳥苷酸(c-di-GMP)調(diào)控下游Clp、Zur和FhrR等全局性轉(zhuǎn)錄因子。為進(jìn)一步研究Fhr R在Xcc中的調(diào)控作用,本研究構(gòu)建了編碼Fhr R蛋白的XC3027基因的整合突變體PK3027。植株試驗(yàn)表明PK3027突變對致病力和過敏(HR)反應(yīng)沒有影響。將連有hrpG、hrpX、hrp B、hrc U和hrp F啟動(dòng)子的p L6GUS報(bào)告質(zhì)粒導(dǎo)入PK3027中,在XCM培養(yǎng)基中檢測gus報(bào)告基因表達(dá),結(jié)果表明XC3027對這些hrp基因的表達(dá)沒有影響。表達(dá)純化Fhr R蛋白后進(jìn)行了凝膠阻滯實(shí)驗(yàn),發(fā)現(xiàn)該轉(zhuǎn)錄調(diào)控蛋白不能直接與hrp X啟動(dòng)子結(jié)合。在Xcc8004中FhrR不參與Ⅲ型分泌系統(tǒng)相關(guān)基因的調(diào)控,并與病原菌致病無關(guān)。
[Abstract]:The Rpf C/Rpf G two-component system of Xanthomonas campestris pv.campestris Xcc) induces the DSF(diffusible signal factor-factor, and regulates the local transcription factors such as Clpsil-Zur and FhrR through the secondary messenger cyclic guanosine monophosphate (c-di-GMP) in the lower reaches of Xanthomonas campestris pv.campestris Xcc. In order to further study the regulatory role of Fhr R in Xcc, an integrated mutant PK302727 of XC3027 gene encoding Fhr R protein was constructed. Plant tests showed that PK3027 mutation had no effect on pathogenicity and hypersensitivity. The expression of gus reporter genes was detected in XCM medium. The results showed that XC3027 had no effect on the expression of these hrp genes. After expression and purification of Fhr R protein, gel arrest assay was carried out. It was found that the transcription regulatory protein could not directly bind to hrp X promoter. FhrR is not involved in the regulation of type 鈪，

本文編號：1914793