本文選題：馬尾松 + 外生菌根　； 參考：《西南大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年05期

【摘要】：利用同源克隆法從菌根化馬尾松中獲得14條磷轉(zhuǎn)運(yùn)蛋白基因片段,對(duì)其中6條(PmP1~PmP6)編碼的氨基酸序列進(jìn)行了比對(duì)和同源性分析,檢測(cè)了不同磷水平下各成員的表達(dá)模式.結(jié)果表明,PmP1~PmP6編碼氨基酸序列具有Pht1磷轉(zhuǎn)運(yùn)蛋白家族的典型特征,成員間相似性達(dá)70%以上.系統(tǒng)分析表明,它們分為5個(gè)亞組,與雙子葉植物的同源性較高,且高度進(jìn)化保守.綜合分析半定量和熒光定量PCR結(jié)果顯示,PmP1~PmP6的表達(dá)受外生菌根誘導(dǎo),且根、莖和針葉中均有表達(dá).未接種菌根真菌時(shí),PmP1~PmP6在針葉中表達(dá)量最高,其次為根;接種后,根中表達(dá)活性升高,其表達(dá)量與生長(zhǎng)環(huán)境中磷水平相關(guān),低磷條件下被高效激活,高磷條件反而抑制其表達(dá).因此,PmP1~PmP6參與菌根化馬尾松體內(nèi)磷的吸收和轉(zhuǎn)運(yùn)過(guò)程.
[Abstract]:Fourteen phosphorous transporter gene fragments were obtained from mycorrhizal Pinus massoniana by homologous cloning method. The amino acid sequences encoded by PmP1 and PmP6 were compared and homologous analyzed, and the expression patterns of each member at different phosphorus levels were detected. The results showed that the amino acid sequence of PmP1 and PmP6 had the typical characteristics of Pht1 phosphorous transporter family, and the similarity among the members was more than 70%. Systematic analysis showed that they were divided into five subgroups with high homology with dicotyledonous plants and highly conserved evolution. The results of semi-quantitative and fluorescence quantitative PCR showed that the expression of PmP1-PmP6 was induced by ectomycorrhizal mycorrhiza, and the expression of PmP6 was found in roots, stems and needles. After inoculation, the expression of PmP1and PmP6 was the highest in the needle, and then in the root. After inoculation, the activity of PmP6 in the root increased, and the expression level was related to the phosphorus level in the growing environment. The expression of PmP1-PmP6 was highly activated under the condition of low phosphorus, but inhibited by the condition of high phosphorus. Therefore, PmP1 and PmP6 are involved in the absorption and transport of phosphorus in the mycorrhizal Pinus massoniana.
【作者單位】： 貴州大學(xué)農(nóng)業(yè)生物工程研究院/生命科學(xué)院/山地植物資源保護(hù)與種質(zhì)創(chuàng)新教育部重點(diǎn)實(shí)驗(yàn)室;貴州大學(xué)林學(xué)院;
【基金】：貴州省科技重大專項(xiàng)(20126011-1) 國(guó)家863子項(xiàng)目(2011AA10020301)
【分類號(hào)】：S791.248

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