本文選題：陽離子聚合物 + 響應(yīng)性��； 參考：《高分子學(xué)報》2017年12期

【摘要】：人類多種疾病都與基因的結(jié)構(gòu)或功能改變密切相關(guān),基因治療已經(jīng)成為改善人類健康的新興醫(yī)學(xué)治療手段,基因治療的關(guān)鍵在于構(gòu)筑高效的基因載體.發(fā)展生物可降解、具有高轉(zhuǎn)染效率和低毒性的陽離子聚合物基因載體已經(jīng)成為當今該領(lǐng)域的主要任務(wù).本文主要介紹了對細胞內(nèi)外的環(huán)境差異和外界刺激具有響應(yīng)性的陽離子聚合物基因載體的合成方法,實現(xiàn)DNA在細胞內(nèi)的有效釋放;構(gòu)筑不同拓撲結(jié)構(gòu)的聚合物,運用聚合物的拓撲結(jié)構(gòu)來調(diào)節(jié)DNA與聚合物組裝復(fù)合體形貌,實現(xiàn)DNA的可控壓縮和提高載體的轉(zhuǎn)染效率;通過兩親性陽離子聚合物的自組裝形成穩(wěn)定的陽離子納米膠束,增加聚合物表面電荷密度,從而有效增強聚合物和DNA結(jié)合能力,實現(xiàn)低正負電荷比條件下的DNA壓縮和基因的高效表達.
[Abstract]:A variety of human diseases are closely related to the structural or functional changes of genes. Gene therapy has become a new medical treatment method to improve human health. The key of gene therapy is to construct efficient gene vectors. The development of biodegradable cationic polymer gene vectors with high transfection efficiency and low toxicity has become a major task in this field. This paper mainly introduces the synthesis method of cationic polymer gene vector which is responsive to the environmental difference inside and outside the cell and external stimuli, realizes the effective release of DNA in cells, constructs the polymer with different topologies, Using the topological structure of polymer to adjust the morphology of DNA / polymer assembly complex, to realize the controllable compression of DNA and to improve the transfection efficiency of the support, and to form stable cationic nanomicelles by self-assembly of amphiphilic cationic polymers. By increasing the surface charge density of the polymer, the binding ability of the polymer to DNA can be effectively enhanced, and the DNA compression and gene expression can be realized under the condition of low positive / negative charge ratio.
【作者單位】： 中國科學(xué)院軟物質(zhì)化學(xué)重點實驗室中國科學(xué)技術(shù)大學(xué)高分子科學(xué)與工程系;
【基金】：國家自然科學(xué)基金(基金號51625305,21474097)資助項目
【分類號】：O631;R450
，

本文編號：1902202