本文選題：鴨坦布蘇病毒 + 核苷酸同源性分析�。� 參考：《動物醫(yī)學進展》2017年09期

【摘要】：山東濰坊地區(qū)某鴨場養(yǎng)殖的21日齡櫻桃谷鴨突然發(fā)病,病鴨主要表現(xiàn)翅腿癱瘓等神經(jīng)癥狀,排黃綠色或黃白色稀糞,病死率5%左右。為了確定發(fā)病鴨群感染病原的種類,首先采集病死鴨肝臟,處理后接種9日齡~11日齡SPF雞胚進行病原分離。其次,對分離的病原RT-PCR擴增和序列測定,MEGA5繪制系統(tǒng)發(fā)育進化樹,DNA Star軟件分析核苷酸同源性。結(jié)果表明,通過SPF雞胚分離到一株坦布蘇病毒(TMUV),命名為TMUV-SDWF。該病毒株基因組全長為10 990nt,編碼1個含3 426個氨基酸的多聚蛋白。系統(tǒng)發(fā)育進化樹分析顯示,TMUV-SDAG毒株屬于Ⅰa分支。核苷酸同源性分析表明,TMUV-SDSG與GenBank上公布的16株不同TMUV分離株核苷酸同源性高達97.0%~99.8%。成功分離鑒定一株鴨坦布蘇病毒并對其進行了遺傳進化分析,為鴨坦布蘇病毒弱毒或滅活疫苗的篩選制備及該病毒分子流行病學的研究奠定了基礎(chǔ)。
[Abstract]:A 21-day-old Cherry Valley duck bred in Weifang area of Shandong Province suddenly developed symptoms such as paralysis of wings and legs and discharge of yellowish green or yellowish-white feces with a fatality rate of about 5%. In order to determine the pathogen of the infected duck, the dead duck liver was collected and inoculated with SPF embryos at 9 and 11 days of age to isolate the pathogen. Secondly, nucleotide homology was analyzed by Star software of phylogenetic tree derived from RT-PCR amplification and sequencing of isolated pathogens. The results showed that a strain of Tambusuvirus was isolated from chicken embryo of SPF and named TMUV-SDWF. The genome length of the virus strain is 10 990 nt, which encodes a polypeptide containing 3 426 amino acids. Phylogenetic tree analysis showed that the TMUV-SDAG strain belonged to branch 鈪，

本文編號：1884076