本文選題：氧化魚(yú)油 + 黏膜　； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年05期

【摘要】：分別提取灌喂氧化魚(yú)油以及魚(yú)油的黃顙魚(yú)胃腸道腸道黏膜的總RNA。將氧化魚(yú)油組、魚(yú)油組總RNA等量混合后,采用RNA-Seq測(cè)序,用Trinity進(jìn)行de novo拼接、組裝,對(duì)單一基因進(jìn)行功能注釋;再將氧化魚(yú)油組、魚(yú)油組總RNA分別測(cè)序、注釋后進(jìn)行基因表達(dá)量分析,并計(jì)算氧化魚(yú)油組對(duì)魚(yú)油組單一基因的差異表達(dá)定量分析,以log_2(OFH/FH)值代表氧化魚(yú)油組相對(duì)于魚(yú)油組基因的差異表達(dá)量�；虿町惐磉_(dá)顯示,氧化魚(yú)油導(dǎo)致黃顙魚(yú)胃腸道黏膜組織中膽固醇、膽汁酸生物合成代謝途徑的酶以及涉及膽固醇和膽汁酸吸收轉(zhuǎn)運(yùn)蛋白基因差異表達(dá)。黃顙魚(yú)胃腸道黏膜中以乙酰輔酶A為原料的膽固醇生物合成途徑關(guān)鍵酶基因差異表達(dá)顯著下調(diào),從細(xì)胞外吸收轉(zhuǎn)運(yùn)膽固醇的主要蛋白基因差異表達(dá)下調(diào),顯示灌喂氧化魚(yú)油導(dǎo)致黃顙魚(yú)胃腸道黏膜膽固醇合成能力下降、從其他組織吸收膽固醇的能力下降,血清膽固醇含量下降4.06%。灌喂氧化魚(yú)油后,黃顙魚(yú)胃腸道黏膜以膽固醇為原料的初級(jí)膽汁酸合成代謝的關(guān)鍵酶差異表達(dá)顯著上調(diào),而膽汁酸轉(zhuǎn)運(yùn)到細(xì)胞外和轉(zhuǎn)運(yùn)到腸腔的載體蛋白基因差異表達(dá)下調(diào),腸道再吸收膽汁酸的轉(zhuǎn)運(yùn)載體蛋白基因差異表達(dá)下調(diào)及肝細(xì)胞從血液吸收轉(zhuǎn)運(yùn)的載體蛋白基因差異表達(dá)上調(diào),而血清膽汁酸含量下降了20.00%。結(jié)果表明,膽汁酸的腸肝循環(huán)發(fā)生障礙,腸腔和血清膽汁酸含量下降、膽汁酸在細(xì)胞出現(xiàn)淤積的趨勢(shì)。
[Abstract]:Total RNA was extracted from gastrointestinal mucosa of Pelteobagrus fulvidraco fed with oxidized fish oil and fish oil, respectively. After equal mixing of total RNA of oxidized fish oil group and fish oil group, RNA-Seq sequencing was used, de novo splicing with Trinity, assembly, function annotation of single gene, and total RNA sequence of oxidized fish oil group and fish oil group were sequenced respectively. After annotation, the gene expression was analyzed, and the differential expression of single gene in the oxidized fish oil group was calculated. The log2OFH / FH value was used to represent the differentially expressed amount of the gene in the oxidized fish oil group as compared with the fish oil group. Gene differential expression showed that oxidized fish oil resulted in differential expression of cholesterol, bile acid biosynthesis pathway, cholesterol and bile acid absorption transporter genes in gastrointestinal mucosa of Pelteobagrus fulvidraco. The differential expression of key enzyme genes in cholesterol biosynthesis pathway from acetyl coenzyme A in the gastrointestinal mucosa of Pelteobagrus fulvidraco was significantly down-regulated, and the differential expression of the main protein genes that absorb and transport cholesterol from the cells was down-regulated. The results showed that the ability of cholesterol synthesis in gastrointestinal mucosa of Pelteobagrus fulvidraco decreased, the ability to absorb cholesterol from other tissues decreased, and the content of serum cholesterol decreased 4.06%. After fed with oxidized fish oil, the differential expression of the key enzymes of primary bile acid synthesis and metabolism in the gastrointestinal mucosa of Pelteobagrus fulvidraco was significantly up-regulated, while the differentially expressed genes of the carrier protein which transported bile acid to the extracellular and intestinal lumen were down-regulated. The differential expression of transporter protein gene of intestinal reabsorbed bile acid was down-regulated and the expression of carrier protein gene of liver cells absorbed and transported from blood was up-regulated, while the content of serum bile acid decreased 20.00%. The results showed that the intestinal and hepatic circulation of bile acids was impaired, the contents of bile acids in intestinal cavity and serum were decreased, and the accumulation of bile acids was observed in the cells.
【作者單位】： 蘇州大學(xué)基礎(chǔ)醫(yī)學(xué)與生物科學(xué)學(xué)院江蘇省水產(chǎn)動(dòng)物營(yíng)養(yǎng)重點(diǎn)試驗(yàn)室;北京營(yíng)養(yǎng)源研究所水產(chǎn)動(dòng)物系統(tǒng)營(yíng)養(yǎng)研究開(kāi)放試驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(K113409511)資助
【分類(lèi)號(hào)】：S965.199
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本文編號(hào)：1883290