本文選題：宮頸腫瘤 + 宮頸癌��； 參考：《山東醫(yī)藥》2017年35期

【摘要】：目的觀察沉默INK4基因座中反義非編碼RNA(ANRILA)對(duì)人宮頸癌細(xì)胞株He La增殖的影響,并探討其機(jī)制。方法取對(duì)數(shù)生長(zhǎng)期He La細(xì)胞分為觀察組和對(duì)照組,分別ANRIL-siRNA、對(duì)照siRNA亂序。轉(zhuǎn)染24、48 h時(shí)采用CCK-8法觀察兩組細(xì)胞增殖情況(結(jié)果以O(shè)D450表示)。轉(zhuǎn)染48 h時(shí)采用熒光定量PCR法檢測(cè)兩組細(xì)胞ANRIL、p21、周期蛋白依賴性激酶6(CDK6)、周期蛋白依賴性激酶2(CDK2)mRNA相對(duì)表達(dá)量,采用CHIP實(shí)驗(yàn)觀察兩組細(xì)胞p21基因H3K27位點(diǎn)三甲基化狀態(tài)。結(jié)果轉(zhuǎn)染24 h觀察組、對(duì)照組OD450分別為0.55±0.08、0.84±0.11,轉(zhuǎn)染72 h觀察組、對(duì)照組OD450分別為1.21±0.12、1.82±0.14。轉(zhuǎn)染24、72 h觀察組OD450均低于對(duì)照組(P0.05)。轉(zhuǎn)染48 h觀察組ANRIL及CDK6、CDK2 mRNA相對(duì)表達(dá)量低于對(duì)照組(P均0.05),p21 mRNA相對(duì)表達(dá)量高于對(duì)照組(P均0.05)。轉(zhuǎn)染48 h兩組細(xì)胞p21基因H3K27位點(diǎn)三甲基化水平觀察組、對(duì)照組分別為1.19±0.08、5.62±0.21,觀察組低于對(duì)照組(P0.05)。結(jié)論沉默ANRIL可抑制宮頸癌He La細(xì)胞的增殖。其機(jī)制是沉默ANRIL可以降低宮頸癌He La細(xì)胞p21基因H3K27位點(diǎn)三甲基化水平,促進(jìn)p21mRNA表達(dá),抑制CDK6、CDK2 mRNA表達(dá),從而抑制宮頸癌He La細(xì)胞增殖。
[Abstract]:Objective to investigate the effect of antisense noncoding RNAs ANRILA in silencing INK4 locus on the proliferation of human cervical cancer cell line he La and its mechanism. Methods he La cells in logarithmic growth phase were divided into two groups: observation group and control group. ANRIL-siRNAs and control siRNA were randomly divided into two groups. The proliferation of two groups of cells was observed by CCK-8 method at 48 h after transfection. The results were expressed as OD450. At 48 h after transfection, the relative expression of ANRIL p21, cyclin dependent kinase 6 CDK6 and cyclin dependent kinase 2(CDK2)mRNA was detected by fluorescence quantitative PCR assay. The trimethylation of H3K27 site of p21 gene in two groups was observed by CHIP assay. Results after 24 h transfection, the OD450 of the control group was 0.55 鹵0.08 鹵0.84 鹵0.11, and the OD450 of the control group was 1.21 鹵0.121.82 鹵0.14 after 72 h transfection. The OD450 of the observation group was lower than that of the control group for 72 h. The relative expression of ANRIL and CDK6 + CDK2 mRNA in the observation group was lower than that in the control group at 48 h after transfection. The relative expression of p21 mRNA in the control group was higher than that in the control group (P < 0.05). The level of H3K27 trimethylation of p21 gene was 1.19 鹵0.08c5.62 鹵0.21 in the control group, which was lower than that in the control group (P 0.05) at 48 h after transfection. Conclusion silencing ANRIL can inhibit the proliferation of helium La cells. The mechanism is that silencing ANRIL can reduce the level of H3K27 trimethylation of p21 gene, promote the expression of p21mRNA and inhibit the expression of CDK6 + CDK2 mRNA in cervical cancer cells, thus inhibit the proliferation of He-La cells.
【作者單位】： 黔西南州人民醫(yī)院;
【分類號(hào)】：R737.33

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