本文選題：菊花 + 低溫��； 參考：《河南農業(yè)大學學報》2017年03期

【摘要】：以秋菊(Chrysanthemum morifolium Ramat.)抗寒品種‘星光燦爛’為材料,利用RT-PCR和RACE方法從葉片中克隆△9硬脂酰-ACP去飽和酶(Stearoyl-ACP desaturase,SAD)基因,命名為CmSAD,Gen Bank登錄號為KC529335。該基因cDNA全長1 203 bp,編碼401個氨基酸,相對分子質量為45.57 k D,等電點為6.48,編碼的氨基酸序列與新疆雪蓮同源性最高(80.29%)。通過對該蛋白進行生物信息學分析得出其沒有跨膜結構,主要存在于葉綠體基質中,N端含有一段包含60個氨基酸的葉綠體轉運肽。通過實時熒光定量PCR研究低溫脅迫下葉片和根系中CmSAD基因的表達量變化,該基因在葉片和根系中均有表達。根系中CmSAD基因的表達量隨著溫度的降低而降低,16℃時表達量最高;葉片中CmSAD表達量隨著溫度的降低先升高再降低,5℃時最高。隨著溫度的降低,菊花葉片和根系中CmSAD基因的表達情況表現(xiàn)出一定的差異。CmSAD基因的表達變化與溫度有關,為低溫脅迫下菊花脂肪酸代謝的分子機理研究提供了基礎。
[Abstract]:Chrysanthemum morifolium Ramat. The gene of 9 stearoyl-ACP desaturase (Stearoyl-ACP desaturase) was cloned from the leaves by RT-PCR and RACE, and named as CmSADGen Bank accession number KC529335. The total length of cDNA is 1 203 BP, encoding 401 amino acids, the relative molecular weight is 45.57 KD, and the isoelectric point is 6.48. The amino acid sequence encoded by this gene has the highest homology with that of Saussurea Sinkiang (80.29). By bioinformatics analysis, it was found that the protein had no transmembrane structure and mainly existed in the chloroplast matrix containing a chloroplast transport peptide containing 60 amino acids. The changes of CmSAD gene expression in leaves and roots under low temperature stress were studied by real-time fluorescence quantitative PCR. The CmSAD gene was expressed in both leaves and roots. The expression of CmSAD gene in roots decreased with the decrease of temperature at 16 鈩，

本文編號：1873232