本文選題：高良姜 + matK基因��； 參考：《中華中醫(yī)藥學(xué)刊》2017年02期

【摘要】：目的:建立基于matK基因的高良姜及其同屬混偽品的分子鑒別方法。方法:采用通用引物對高良姜樣品matK基因進(jìn)行PCR擴(kuò)增和雙向測序,并從Gen Bank下載10種同屬混偽品的matK基因序列。采用Clustal X、MEGA軟件進(jìn)行序列比對分析、計(jì)算遺傳距離以及構(gòu)建聚類樹。結(jié)果:獲得的高良姜及其同屬混偽品matK基因序列長度為732 bp,變異位點(diǎn)41個(gè)。高良姜種內(nèi)遺傳距離為0.000,與混偽品之間的最小遺傳距離為0.003�；趍atK序列構(gòu)建的系統(tǒng)發(fā)生樹顯示高良姜樣品聚在一起,能直觀地與混偽品區(qū)分。結(jié)論:matK基因可以有效地鑒別高良姜及其同屬混偽品。
[Abstract]:Objective: to establish a molecular identification method for Ginger and its concoctions based on matK gene. Methods: the matK gene of Ginger was amplified by PCR and bidirectional sequencing with universal primers, and 10 matK gene sequences were downloaded from Gen Bank. Sequence alignment analysis, genetic distance calculation and clustering tree construction were carried out by using Clustal Xomega software. Results: the length of matK gene was 732 BP with 41 mutation sites. The intraspecific genetic distance of Ginger was 0.000, and the minimum genetic distance between Ginger and its adulterants was 0.003. The phylogenetic tree constructed based on matK sequence showed that the samples of alpinia officinalis were gathered together and could be directly distinguished from the adulterates. ConclusionThe gene of 1: matk can be used to identify alpinia officinalis and its adulterates.
【作者單位】： 廣東醫(yī)科大學(xué);廣州中醫(yī)藥大學(xué)中藥資源科學(xué)與工程研究中心;嶺南中藥資源教育部重點(diǎn)實(shí)驗(yàn)室;
【分類號】：R284.1

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1 生書晶;嚴(yán)萍;鄭傳進(jìn);趙樹進(jìn);;何首烏及其常見混淆品的matK基因序列分析及鑒別[A];共鑄醫(yī)藥學(xué)術(shù)新文明——2012年廣東省藥師周大會論文集[C];2012年

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