本文選題：玉米 + 性別決定��； 參考：《南京農(nóng)業(yè)大學(xué)學(xué)報》2017年05期

【摘要】：[目的]TS2(tasselseed2)是玉米性別決定中的一個關(guān)鍵基因,國內(nèi)外對其進(jìn)行了多方面的研究,但其生理學(xué)功能至今仍然不清楚。本研究通過TS2基因的表達(dá)模式來闡明TS2的表達(dá)受什么信號物質(zhì)刺激,從而推測TS2通過何種生理過程或信號轉(zhuǎn)導(dǎo)途徑來實現(xiàn)其在玉米性別決定中的作用。[方法]以玉米自交系B73苗期的葉片、莖尖、根,性別分化時期的穗位葉、雄蕊,開花期的雌蕊、節(jié)間、氣生根、花絲為試驗材料,在V3期(3葉1心期)噴施100μmol·L-1的GA、NAA、ABA、ACC、JA和2.5 mmol·L-1的SA溶液(含0.01%Tween-20),分別于激素處理后0、6、12、24、48、72 h時剪取第3葉中段為樣本,采用熒光定量PCR測定TS2受激素誘導(dǎo)的表達(dá)量。[結(jié)果]TS2基因在已檢測的各組織中都有一定量的表達(dá)。在葉片中TS2的表達(dá)從苗期到開花期逐漸增加,V8期(8葉1心期)葉片內(nèi)的TS2表達(dá)量達(dá)到最高峰,以后逐漸下降。苗期(V1~V7)(1葉1心期到7葉1心期)莖尖中TS2的表達(dá)量一直很低,但到V8期,植株莖尖開始性別分化時,TS2的表達(dá)量迅速提高20多倍。雄穗、雌穗內(nèi)TS2的表達(dá)量高于葉片或其他營養(yǎng)器官。植物激素GA、SA、NAA抑制TS2的表達(dá),ABA、ACC、JA促進(jìn)TS2的表達(dá),但以JA對TS2的表達(dá)影響最大,JA處理后24 h,TS2的表達(dá)量提高了84倍。[結(jié)論]植物性別決定基因TS2的表達(dá)主要受植物生殖發(fā)育過程和植物激素茉莉酸信號的調(diào)控,預(yù)示著TS2的生理功能與植株體內(nèi)茉莉酸信號轉(zhuǎn)導(dǎo)途徑密切相關(guān)。
[Abstract]:[objective] TS2tasselseed2 is a key gene in sex determination of maize, which has been studied in many fields at home and abroad, but its physiological function is still unclear. In this study, the expression pattern of TS2 gene was used to elucidate what signal substance stimulates the expression of TS2, so as to speculate the physiological process or signal transduction pathway through which TS2 plays a role in the sex determination of maize. [methods] leaves, stem tips, roots, ear leaves, stamens, pistil, internodes, aerial roots and filaments of maize inbred line B73 at seedling stage were used as experimental materials. 100 渭 mol L-1 GANAA ACCJA and 2.5 mmol L-1 SA solution (containing 0.01 Tween-20) were sprayed on the third leaf of stage V3. The middle segment of the third leaf was cut for 72 h after steroid treatment, and the expression of TS2 induced by hormone was determined by fluorescence quantitative PCR. [results] TS2 gene was expressed in all tissues detected. The expression of TS2 in leaves increased gradually from seedling stage to flowering stage. In seedling stage, the expression of TS2 in stem tip was very low from 1 heart stage to 7 leaf 1 heart stage, but at V8 stage, the expression of Ts2 increased more than 20 times when the stem tip began to differentiate. The expression of TS2 in male ear and female ear was higher than that in leaves or other vegetative organs. The plant hormone GAA SAA inhibited the expression of TS2. ACC-JA promoted the expression of TS2, but JA had the greatest effect on the expression of TS2. 24 h after JA treatment, the expression of TS2 increased 84 times. [conclusion] the expression of plant sex determinant gene TS2 is mainly regulated by plant reproductive development and plant hormone jasmonic acid signal, indicating that the physiological function of TS2 is closely related to the signal transduction pathway of jasmonic acid in plant.
【作者單位】： 南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)資源創(chuàng)新國家重點實驗室;
【基金】：國家自然科學(xué)基金項目(31571580) 南京農(nóng)業(yè)大學(xué)人才引進(jìn)項目(KYRC201404) 中央高�；究蒲袠I(yè)務(wù)費專項資金(KYTZ201402) 江蘇省優(yōu)勢學(xué)科資助項目
【分類號】：S513

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