本文選題：食管腫瘤 + 樹突狀細胞��； 參考：《河北醫(yī)科大學學報》2016年12期

【摘要】：目的研究IL-27基因轉染的樹突狀細胞(dendritic cell,DC)活化CTL體內誘導食管癌細胞凋亡的影響。方法通過在裸鼠的移植瘤周注射食管癌細胞抗原致敏、IL-27基因修飾DC(DC_(IL-27+Ag))活化的特異CTL,采用流式細胞術檢測細胞凋亡水平,熒光染料羅丹明123染色檢測細胞線粒體膜電位水平并檢測caspase-3蛋白表達水平。結果 5組之間凋亡率、膜電位水平、caspase-3表達差異均有統(tǒng)計學意義(P0.05):DC_(IL-27+Ag)、DCIL-27、DCnaive、Tnaive組凋亡率、caspase-3表達均高于PBS組,膜電位水平低于PBS組;DC_(IL-27+Ag)、DCIL-27、DCnaive組凋亡率、caspase-3表達均高于Tnaive組,膜電位水平低于Tnaive組;DC_(IL-27+Ag)、DC_(IL-27)組凋亡率、caspase-3表達均高于DCnaive組,膜電位水平低于DCnaive組;DC_(IL-27+Ag)組凋亡率、caspase-3表達均高于DCIL-27組,膜電位水平低于DCIL-27組。結論在荷瘤小鼠體內,經(jīng)食管癌細胞抗原致敏、IL-27基因修飾的DC可活化特異性CTL產(chǎn)生較強的細胞毒作用,其機制可能是CTL通過降低食管癌細胞線粒體膜電位,啟動內源性線粒體凋亡途徑,激活caspase-3蛋白,從而誘導細胞凋亡。
[Abstract]:Objective to study the effect of IL-27 gene transfected dendritic cells (DC) on apoptosis of esophageal carcinoma cells induced by CTL. Methods specific CTLs were injected into the xenografts of nude mice, which were sensitized with IL-27 gene and modified with DC(DC_(IL-27. Flow cytometry was used to detect the level of apoptosis. The mitochondrial membrane potential and the expression of caspase-3 protein were detected by Rhodamine 123 staining. Results there were significant differences in the expression of caspase-3 and apoptosis between the five groups. The expression of caspase-3 was significantly higher in DCIL-27 / Ag-DCIL27 / Tnaive group than in PBS group, and the level of membrane potential was lower than that in PBS group. The expression of caspase-3 in DCIL-27 / DCIL-27DCnaive group was significantly higher than that in Tnaive group, and the expression of caspase-3 in DCIL-27 / DCnaive group was significantly higher than that in PBS group. The level of membrane potential was lower than that of Tnaive group. The expression of caspase-3 and caspase-3 in DCS group was higher than that in DCnaive group, and the expression of caspase-3 in DCnaive group was higher than that in DCIL-27 group, and the membrane potential level was lower than that in DCIL-27 group. Conclusion in tumor-bearing mice, IL-27 gene modified DC can activate specific CTL to produce strong cytotoxic effect, and the mechanism may be that CTL can reduce mitochondrial membrane potential of esophageal cancer cells. Activation of endogenous mitochondrial apoptosis pathway, activation of caspase-3 protein, thereby inducing apoptosis.
【作者單位】： 河北省隆堯縣醫(yī)院胸外科;河北省涉縣醫(yī)院病理科;冀中能源峰峰集團有限公司總醫(yī)院外科;冀中能源峰峰集團有限公司總醫(yī)院皮膚性病科;河北醫(yī)科大學第四醫(yī)院胸外科;
【基金】：河北省醫(yī)學科學研究重點課題(20110136)
【分類號】：R735.1

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