本文選題：剛毛檉柳 + DREB轉(zhuǎn)錄因子　； 參考：《植物研究》2017年01期

【摘要】：DREB蛋白能特異地與DRE(dehydration responsive element)順式作用元件結(jié)合,從而調(diào)控下游多個與逆境相關(guān)基因的表達,在植物對多種逆境脅迫的應答反應中起重要調(diào)節(jié)作用。為了研究剛毛檉柳ThDREB基因是否具有抗逆功能,將Th DREB基因插入到酵母表達載體p YES2中構(gòu)建成重組載體,轉(zhuǎn)入釀酒酵母(Saccharomyces cerevisiae)中獲得重組型酵母。分別比較轉(zhuǎn)ThDREB基因酵母和轉(zhuǎn)空載體對照酵母在山梨醇、H_2O_2、CdCl_2、NaCl、Na_2CO_3、MgCl_2、-20℃脅迫處理之后的存活能力。結(jié)果顯示,ThDREB基因能有效提高轉(zhuǎn)基因酵母的抗干旱、鹽、堿、氧化、重金屬及低溫脅迫的能力,表明Th DREB基因可能參與了檉柳多種抗逆調(diào)控過程。
[Abstract]:DREB protein can specifically bind to DRE(dehydration responsive element-cis-acting elements, thus regulating the expression of many downstream stress-related genes, which plays an important role in the response of plants to various stresses. In order to study the stress resistance of Tamarix chinensis ThDREB gene, the Th DREB gene was inserted into the yeast expression vector p YES2 to construct the recombinant vector, and then transferred into Saccharomyces cerevisiae to obtain the recombinant yeast. The viability of transgenic yeast with ThDREB gene was compared with that of vector yeast after stress treatment at 20 鈩，

本文編號：1845631