本文選題：高寒牧區(qū) + 藏綿羊　； 參考：《江西農(nóng)業(yè)大學(xué)學(xué)報(bào)》2017年03期

【摘要】：為分析高寒牧區(qū)藏綿羊ANGPTL4基因多態(tài)性,應(yīng)用PCR-SSCP與測(cè)序結(jié)合的方法,對(duì)391只藏綿羊ANGPTL4基因第3內(nèi)含子、第6外顯子進(jìn)行多態(tài)性檢測(cè)。經(jīng)擴(kuò)增,分別獲得大小為280 bp和289 bp的2個(gè)片段。ANGPTL4基因第3內(nèi)含子發(fā)現(xiàn)AA、BB和AB 3種基因型,各基因型頻率分別為0.648 8、0.313 0和0.038 2,在C.400+90處發(fā)生G/T的突變,多態(tài)信息含量為0.265 6,呈中度多態(tài)。ANGPTL4基因第6外顯子發(fā)現(xiàn)AA和AB2種基因型,基因型頻率分別為0.961 8和0.038 2,在C.837處發(fā)生C/T的突變,為錯(cuò)義突變,并導(dǎo)致P.279處蘇氨酸變?yōu)榻z氨酸,多態(tài)信息含量為0.036 9,為低度多態(tài)。對(duì)藏綿羊ANGPTL4基因的多態(tài)性進(jìn)行分析,發(fā)現(xiàn)的2處突變位點(diǎn)可作為藏綿羊品種改良以及種質(zhì)資源保護(hù)的潛在分子標(biāo)記。
[Abstract]:In order to analyze the polymorphism of ANGPTL4 gene in Tibetan sheep in alpine pastoral area, the polymorphism of intron 3 and exon 6 of ANGPTL4 gene in 391 Tibetan sheep was detected by PCR-SSCP and sequencing. Two fragments, 280bp and 289bp, were amplified and three genotypes were found in intron 3 of the ANGPTL4 gene. The frequencies of each genotype were 0.648 and 0.038 2, respectively. The mutation of G / T occurred at C.40090. The polymorphic information content was 0.265, which showed that AA and AB2 genotypes were found in exon 6 of medium polymorphic .ANGPTL4 gene. The genotype frequencies were 0.961 8 and 0.038 2, respectively. The mutation of C / T occurred at C.837, which was missense mutation and resulted in the conversion of threonine to serine at P.279. The polymorphic information content was 0.036, which was low polymorphic. By analyzing the polymorphism of ANGPTL4 gene in Tibetan sheep, we found that the two mutation sites could be used as potential molecular markers for the improvement of Tibetan sheep breeds and the protection of germplasm resources.
【作者單位】： 甘肅農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)技術(shù)學(xué)院/甘肅省草食動(dòng)物生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31260546) 國(guó)家科技支撐計(jì)劃項(xiàng)目(2012BAD13B05)~~
【分類號(hào)】：S826

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1 李文浩;羔羊肉生產(chǎn)雜交組合篩選及藏綿羊ANGPTL4基因多態(tài)性分析[D];甘肅農(nóng)業(yè)大學(xué);2016年

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本文編號(hào)：1844632