本文選題：豬 + IGFBP�。� 參考：《中國(guó)畜牧雜志》2017年07期

【摘要】：胰島素樣生長(zhǎng)因子結(jié)合蛋白(Insulin-like Growth Factor Binding Proteins,IGFBPs)與動(dòng)物的生長(zhǎng)發(fā)育有著密切的聯(lián)系,為研究IGFBP3的多態(tài)性與豬生長(zhǎng)性狀之間的關(guān)系,本實(shí)驗(yàn)采用PCR-RFLP方法對(duì)大白豬、杜洛克及長(zhǎng)白豬IGFBP3基因內(nèi)含子2的多態(tài)性進(jìn)行分析,并且研究了IGFBP3不同基因型對(duì)體長(zhǎng)、體高、瘦肉率、活體背膘、肌肉厚度、0~100 kg日增重、30~100 kg日增重及100 kg體重日齡等主要生長(zhǎng)性狀的影響。結(jié)果表明:IGFBP3基因內(nèi)含子2共417 bp,在3個(gè)豬群中有1個(gè)A307G多態(tài)位點(diǎn)與豬的生長(zhǎng)性狀有關(guān),并且都是AG基因型占優(yōu)勢(shì),杜洛克與長(zhǎng)白豬在該位點(diǎn)上偏離Hardy-Weinberg平衡(P0.010);3個(gè)品種豬群IGFBP3基因的多態(tài)性信息含量(PIC)均表現(xiàn)為中度多態(tài)(0.34~0.37);IGFBP3基因的GG基因型對(duì)大白豬的體高(P=0.068)、瘦肉率(P=0.058)的影響較大,對(duì)杜洛克豬的瘦肉率(P=0.099)、肌肉厚度(P=0.079)的影響較大,對(duì)長(zhǎng)白豬的0~100 kg日增重的影響均接近顯著水平(P=0.08)。GG基因型豬具有體高、瘦肉率高和生長(zhǎng)較快的特點(diǎn),為以后豬的育種和選種提供參考。
[Abstract]:Insulin-like Growth Factor Binding proteins (IGFBPs) is closely related to the growth and development of animals. In order to study the relationship between IGFBP3 polymorphism and pig growth traits, PCR-RFLP method was used in this study. The polymorphism of intron 2 of IGFBP3 gene in Duroc and Landrace pigs was analyzed, and the effects of different genotypes of IGFBP3 on body length, body height, lean meat rate and backfat in vivo were studied. The effects of muscle thickness of 100 kg / day and 100kg / kg daily gain on the main growth traits. The results showed that the intron 2 of the 1: IGFBP3 gene was 417bp. one A307G polymorphic locus in three breeds was related to the growth traits of pigs, and all of them were the dominant genotype of AG. Duroc and Landrace deviated from the Hardy-Weinberg balance at this locus P0.0101.The polymorphism information content of IGFBP3 gene in the three breeds showed that the GG genotype of IGFBP3 gene had a great influence on the body height of large white pigs, and the lean meat percentage was P0.058. The effect on lean meat rate and muscle thickness of Duroc pigs was greater, and the effects on daily gain of 0 ~ 100kg / kg of Landrace pigs were close to the significant level. The genotype pigs had the characteristics of high body height, high lean meat rate and fast growth. To provide reference for future pig breeding and selection.
【作者單位】： 青島農(nóng)業(yè)大學(xué)動(dòng)物科技學(xué)院;
【基金】：資助項(xiàng)目:山東省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系生豬創(chuàng)新團(tuán)隊(duì)(SDAI T-08-13)
【分類號(hào)】：S828.2

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