本文選題：嗜熱果糖苷酶 + 基因克隆�。� 參考：《中國食品添加劑》2017年04期

【摘要】：從嗜熱菌Thermotoga neapolitana DSM4359得到嗜熱果糖苷酶基因片段,以p ET-28a(+)為表達(dá)載體,在Escherichia.coil BL21(DE3)中高效表達(dá)。由SDS-PAGE檢測(cè)可得構(gòu)建后嗜熱果糖苷酶的分子量約為60 KDa。通過單因素試驗(yàn)和響應(yīng)面法對(duì)該嗜熱果糖苷酶的產(chǎn)酶培養(yǎng)基進(jìn)行優(yōu)化選擇,最終確定最佳產(chǎn)酶培養(yǎng)基為:蔗糖10.11 g/L,硝酸銨12.30 g/L,安琪酵母浸粉FM905 8.24 g/L,MgSO_4·7H_2O 5.61 mmol/L,NaCl 10 g/L。相比優(yōu)化前,經(jīng)優(yōu)化后的培養(yǎng)基所產(chǎn)嗜熱果糖苷酶酶活提高了近2.1倍。達(dá)到了提高嗜熱果糖苷酶表達(dá)量的目的。
[Abstract]:The thermophilic fructosidase gene fragment was obtained from thermophilic bacteria Thermotoga neapolitana DSM4359 and was highly expressed in Escherichia.coil BL21DE3 using pET-28a () as expression vector. The molecular weight of the constructed thermophilic glucosidase was about 60 KDa. detected by SDS-PAGE. Single factor test and response surface method were used to optimize the enzyme production medium of the thermophilic fructosidase. The optimum medium was determined as follows: sucrose 10.11 g / L, ammonium nitrate 12.30 g / L, An Qi yeast extract FM905 8.24 g / L, MgSO4 7H_2O 5.61 mmol / L NaCl 10 g / L. Compared with that before optimization, the thermotropic fructosidase activity of the optimized medium was increased by nearly 2.1 times. The purpose of increasing the expression of thermophilic fructosidase was achieved.
【作者單位】： 吉林農(nóng)業(yè)大學(xué)食品科學(xué)與工程學(xué)院;
【基金】：吉林省教育廳“十二五”科學(xué)技術(shù)研究項(xiàng)目(吉教科合字[2015]第207號(hào)) 吉林省科技廳科技創(chuàng)新人才培育計(jì)劃項(xiàng)目(項(xiàng)目編號(hào):20140519011JH)
【分類號(hào)】：Q55;Q78
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本文編號(hào)：1843899