發(fā)布時(shí)間：2018-05-01 10:35

  本文選題：奈達(dá)鉑 + 順鉑　； 參考：《中華腫瘤防治雜志》2016年19期

【摘要】：目的奈達(dá)鉑(Nedaplatin,NDP)是第2代鉑類化合物,抗腫瘤的機(jī)制與順鉑(Cisplatin,DDP)相同,其腎臟和胃腸道等不良反應(yīng)均小于DDP,且無(wú)需水化,使用方便。本研究探討NDP對(duì)裸鼠MGC-803胃癌移植瘤的抑制作用及對(duì)p53基因表達(dá)的影響。方法制備MGC-803胃癌荷瘤裸鼠模型,MGC-803人胃癌細(xì)胞株接種裸鼠約1周時(shí)間,待腫瘤生長(zhǎng)至直徑約5~9mm隨機(jī)分為空白對(duì)照組、DDP組和NDP低、中和高劑量組,每組8只。分別予以腹腔注射生理鹽水、順鉑、不同劑量奈達(dá)鉑(1.0、2.0和3.0 mg/kg),采用蛋白質(zhì)印跡法及RT-PCR法檢測(cè)瘤體中p53基因的表達(dá)。結(jié)果空白對(duì)照組、DDP組和NDP低、中和高劑量組p53mRNA表達(dá)量分別為0.297±0.024、0.892±0.098、0.444±0.016、0.561±0.034和0.585±0.021。空白對(duì)照組、DDP組和NDP低、中和高劑量組p53蛋白表達(dá)量分別為0.295±0.027、0.541±0.032、0.316±0.034、0.408±0.028和0.525±0.041。p53mRNA及蛋白在DDP組和NDP組相對(duì)表達(dá)量均高于空白對(duì)照組,P0.05。與DDP組對(duì)比,p53mRNA在NDP組均降低,P0.05;p53蛋白表達(dá)在NDP低劑量組和NDP中劑量組中均降低(P0.05),而在NDP高劑量組中表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義,P0.05。NDP不同濃度化療組間比較,p53mRNA及蛋白表達(dá)水平呈量效關(guān)系,即隨著藥物劑量增大,其表達(dá)量增高。結(jié)論NDP能增加MGC-803胃癌荷瘤裸鼠腫瘤組織p53mRNA及蛋白表達(dá)水平,但與DDP相比未見(jiàn)明顯優(yōu)勢(shì)。
[Abstract]:Objective Nedaplatin (NDP) is the second generation platinum compound with the same antitumor mechanism as cisplatin Cisplatin (DDP). The adverse reactions in kidney and gastrointestinal tract of Nedaplatin NDP are less than those of DDP, and they need no hydration and are easy to use. The aim of this study was to investigate the inhibitory effect of NDP on xenografts of MGC-803 gastric carcinoma in nude mice and its effect on p53 gene expression. Methods Human gastric cancer cell line MGC-803 was inoculated into nude mice for about 1 week. When the tumor grew to a diameter of about 5~9mm, it was randomly divided into two groups: control group (n = 8) and control group (n = 8) with low, medium and high doses of NDP. Intraperitoneal injection of normal saline, cisplatin, and different doses of nidaplatin 1.0 mg / kg and 3.0 mg / kg 路kg ~ (-1) respectively. The expression of p53 gene was detected by Western blotting and RT-PCR assay. Results the expression of p53mRNA in the control group was lower than that in the control group. The expression of p53mRNA in the middle and high dose groups was 0.297 鹵0.024 鹵0.098 鹵0.444 鹵0.016, 0.561 鹵0.034 and 0.585 鹵0.021, respectively. The relative expression of p53 protein in DDP group and NDP group was significantly higher than that in DDP group and NDP group. The expression levels of p53 protein in the control group were 0.295 鹵0.027, 0.541 鹵0.032, 0.316 鹵0.034, 0.408 鹵0.028 and 0.525 鹵0.041.p53mRNA, respectively. The relative expression levels of p53 protein in the DDP group and the NDP group were higher than those in the control group. Compared with DDP group, the expression of p53 protein in NDP group was significantly lower than that in NDP group. The expression of p53 protein in NDP low dose group and NDP medium dose group was decreased, but there was no significant difference in NDP high dose group. There was a dose-effect relationship between different concentrations of P0.05.NDP chemotherapy group and the expression level of p53mRNA and protein. That is, with the increase of drug dose, its expression increased. Conclusion NDP can increase the expression of p53mRNA and protein in tumor tissues of nude mice bearing MGC-803 gastric cancer, but there is no obvious advantage compared with DDP.
【作者單位】： 萊蕪市人民醫(yī)院腫瘤科;萊蕪市人民醫(yī)院普外科;萊蕪市婦幼保健院;
【分類號(hào)】：R735.2

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