本文選題：新疆野扁桃 + 自交不親和性�。� 參考：《經(jīng)濟(jì)林研究》2017年02期

【摘要】：為給野扁桃的遺傳改良和自交不親和機(jī)制的進(jìn)一步研究提供理論依據(jù),以新疆野扁桃花藥為試材,利用RT-PCR和RACE技術(shù)克隆野扁桃自交不親和性花粉特異性決定因子編碼基因SFB全長序列。采用BLAST進(jìn)行序列比對(duì),ORF Finder尋找開放閱讀框,CDD分析蛋白保守結(jié)構(gòu)域,Prot Param分析蛋白理化性質(zhì),TMHMM預(yù)測(cè)蛋白跨膜區(qū),Signal P預(yù)測(cè)蛋白信號(hào)肽,Sub Loc預(yù)測(cè)蛋白亞細(xì)胞定位,SOPMA分析蛋白二級(jí)結(jié)構(gòu),DANMAN進(jìn)行氨基酸多序列比對(duì),MEGA6進(jìn)行系統(tǒng)進(jìn)化分析,Prot Fun預(yù)測(cè)蛋白功能。結(jié)果表明:克隆到的Pt SFB16基因和Pt SFB17基因?qū)儆贔-box基因家族,與其它多種植物的SLF/SFB基因的序列相似度為88%,推導(dǎo)的氨基酸序列均具有F-box蛋白典型結(jié)構(gòu);Pt SFB16基因ORF長1 146 bp,編碼381個(gè)氨基酸,Pt SFB17基因ORF長1 131 bp,編碼376個(gè)氨基酸;預(yù)測(cè)2個(gè)SFB蛋白均為略顯親水性、不穩(wěn)定的細(xì)胞質(zhì)蛋白,二級(jí)結(jié)構(gòu)均以α-螺旋,延伸鏈和無規(guī)則卷曲為主,SFB/SLF蛋白在薔薇科李屬植物中具有較高的系統(tǒng)進(jìn)化一致性,種間同源性可能大于種內(nèi)同源性,SFB蛋白可能的功能主要有輔助因子生物合成、能量代謝和裂合酶。
[Abstract]:In order to provide theoretical basis for the further study of genetic improvement and self-incompatibility mechanism of wild almond, the anthers of Xinjiang wild almond were used as test materials. RT-PCR and RACE techniques were used to clone the full-length SFB encoding gene of self-incompatibility pollen specific determinant gene (SFB) in wild almond. Sequence alignment using BLAST Finder to search for open reading frame to analyze protein conserved domain Prot Param analysis of protein physicochemical properties and prediction of protein transmembrane signal peptide Sub Loc protein subcellular localization White secondary structure (DANMAN) was used for amino acid multiple sequence alignment. Phylogenetic analysis of MEGA6 was performed to predict the protein function by Prot Fun. The results showed that the cloned Pt SFB16 gene and Pt SFB17 gene belonged to the F-box gene family. The sequence similarity of SLF/SFB gene with other plants is 88. The deduced amino acid sequences have the typical structure of F-box protein, the ORF length of Pt SFB16 gene is 1 146 BP, the length of Pt SFB17 gene ORF is 1 131 BP, and the deduced amino acid sequence is 376 amino acids. It was predicted that the two SFB proteins were hydrophilic and unstable, and the secondary structures were 偽 -helix, extended chain and irregular curl, which had high phylogenetic consistency in the genus Rosaceae. The main functions of SFB protein may be related to biosynthesis, energy metabolism and cleavage synthase.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)林學(xué)與園藝學(xué)院;新疆農(nóng)業(yè)大學(xué)果樹學(xué)新疆特色果樹研究中心;新疆農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目“新疆野扁桃自交不親和性的分子機(jī)制研究”(31260465) 林業(yè)公益性行業(yè)科研專項(xiàng)“新疆特色林果樹提質(zhì)增效關(guān)鍵技術(shù)研究與示范之專題——新疆特色林果優(yōu)良品種選育與推廣”(201304701-1) 新疆維吾爾自治區(qū)果樹學(xué)重點(diǎn)學(xué)科(201007)
【分類號(hào)】：S662.9

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本文編號(hào)：1802687