本文選題：子癇前期 + 人類(lèi)白細(xì)胞抗原G��； 參考：《青海大學(xué)》2017年碩士論文

【摘要】：目的:通過(guò)研究青海地區(qū)藏漢民族子癇前期患者與HLA-G基因非編碼區(qū)多態(tài)性的相關(guān)性,從而篩選出青海地區(qū)藏漢民族孕婦易感子癇前期所對(duì)應(yīng)的HLA-G多態(tài)性位點(diǎn),為子癇前期的病因?qū)W研究提供依據(jù),指導(dǎo)臨床對(duì)子癇前期的預(yù)防。方法:收集2015年4月到2016年10月期間在青海大學(xué)附屬醫(yī)院產(chǎn)科分娩的藏漢民族正常妊娠孕婦及子癇前期患者的血液標(biāo)本共348例,采用DNA提取、聚合酶聯(lián)反應(yīng)技術(shù)、瓊脂糖凝膠電泳及DNA測(cè)序等方法找出HLA-G基因非編碼區(qū)存在的多態(tài)性位點(diǎn),進(jìn)而通過(guò)相應(yīng)的統(tǒng)計(jì)學(xué)方法分析四組間各位點(diǎn)的等位基因及基因型分布是否存在差異,最后得出相應(yīng)的結(jié)論。結(jié)果:四組間HLA-G的Exon8 14bp插入/缺失多態(tài)性分布差異無(wú)統(tǒng)計(jì)學(xué)意義,而將基因型合并后發(fā)現(xiàn)II基因型和ID/DD基因型分布在漢族正常妊娠組和藏族正常妊娠組之間差異具有統(tǒng)計(jì)學(xué)意義;在HLA-G的5-URR區(qū)域發(fā)現(xiàn)6個(gè)單核苷酸多態(tài)性位點(diǎn),其中2個(gè)(-289、-274)位點(diǎn)多態(tài)性在四組間分布差異具有統(tǒng)計(jì)學(xué)意義,兩兩比較后-289位點(diǎn)的等位基因分布在漢族正常妊娠組和漢族子癇前期組之間有統(tǒng)計(jì)學(xué)差異,-274位點(diǎn)的基因型和等位基因在漢族正常妊娠組和藏族正常妊娠組之間有統(tǒng)計(jì)學(xué)差異,-289/-274位點(diǎn)聯(lián)合基因型在漢族正常妊娠組和漢族子癇前期組之間有統(tǒng)計(jì)學(xué)差異。結(jié)論:HLA-G基因Exon8 14bp插入/缺失位點(diǎn)多態(tài)性和HLA-G基因5-URR的-274位點(diǎn)多態(tài)性可能與民族差異有關(guān),而與青海地區(qū)藏漢族孕婦子癇前期的易感性的關(guān)系還需進(jìn)一步擴(kuò)大樣本數(shù)量來(lái)探討;HLA-G基因5-URR的-289位點(diǎn)多態(tài)性可能與青海地區(qū)漢族子癇前期的易感性有關(guān);HLA-G基因5-URR的-289/-274位點(diǎn)聯(lián)合多態(tài)性可能與青海地區(qū)漢族子癇前期的易感性有關(guān)。
[Abstract]:Objective: to study the association between preeclampsia and non-coding region polymorphism of HLA-G gene in Tibetan and Han nationality preeclampsia patients in Qinghai area, and to screen out the HLA-G polymorphism corresponding to preeclampsia in Tibetan and Han nationality pregnant women in Qinghai area. To provide the basis for the etiological study of preeclampsia and to guide the clinical prevention of preeclampsia. Methods: from April 2015 to October 2016, the blood samples of normal pregnant women and preeclampsia were collected from 348 pregnant women and preeclampsia patients who were delivered by obstetrics in the affiliated Hospital of Qinghai University. DNA extraction and polymerase chain reaction (PCR) were used. Agarose gel electrophoresis and DNA sequencing were used to identify the polymorphic loci in the non-coding region of the HLA-G gene, and then the alleles and genotypes of the four groups were analyzed by statistical methods. Finally, the corresponding conclusions are drawn. Results: there was no significant difference in the distribution of Exon8 14bp insertion / deletion polymorphism in HLA-G among the four groups. It was found that the distribution of genotype II and ID/DD genotype was statistically significant between normal pregnancy group of Han nationality and normal pregnancy group of Tibetan nationality, and six single nucleotide polymorphism loci were found in 5-URR region of HLA-G. There were significant differences in the distribution of two loci polymorphism among the four groups. The allele distribution of -289 locus was significantly different between normal pregnancy group and preeclampsia group of Han nationality. The genotype and allele of -274 locus were found in normal pregnancy group of Han nationality and normal pregnancy group of Tibetan nationality. Statistical difference was found between Han normal pregnancy group and Han preeclampsia group. Conclusion the polymorphism of insertion / deletion locus of Exon8 14bp of HLA-G gene and the polymorphism of 5-URR locus of HLA-G gene may be related to ethnic differences. The relationship between preeclampsia and preeclampsia in Tibetan and Han women in Qinghai area needs to be further expanded to explore the relationship between HLA-G gene 5-URR polymorphism and preeclampsia preeclampsia preeclampsia (5-URR) gene in Qinghai Han nationality. The-289%-274 locus polymorphism may be associated with preeclampsia susceptibility in the Han nationality in Qinghai.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R714.244

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 夏小艷;徐潔歡;李耀軍;;sHLA-G檢測(cè)在妊娠早期流產(chǎn)中的研究進(jìn)展[J];生物技術(shù)世界;2016年05期

2 王亞琴;肖靜;吳義軍;羅正春;梅軍華;;人白細(xì)胞抗原-G在胎膜早破患者血清及胎膜中的表達(dá)及臨床意義[J];中國(guó)婦幼保健;2016年05期

3 張若鵬;張麗蓉;朱莉;吳睿;于成和;邵華;;sHLA-G主動(dòng)免疫治療復(fù)發(fā)性流產(chǎn)的大鼠實(shí)驗(yàn)研究[J];云南醫(yī)藥;2016年01期

4 單士剛;包永芬;劉超;;HLA-G基因多態(tài)性與疾病關(guān)聯(lián)的研究進(jìn)展[J];現(xiàn)代免疫學(xué);2015年06期

5 張旱梅;尹宗智;李惠萍;;HLA-G基因14 bp插入/缺失多態(tài)性與原因不明復(fù)發(fā)性流產(chǎn)關(guān)聯(lián)的Meta分析[J];中國(guó)婦幼保健;2015年23期

6 韋瑋;余波;章如松;陸珍鳳;王建東;石群立;;卵巢漿液性癌中HLA-G的表達(dá)及臨床意義[J];臨床與實(shí)驗(yàn)病理學(xué)雜志;2015年05期

7 趙俊玲;王若崢;;HPV感染及HLA多態(tài)性與宮頸癌相關(guān)性研究進(jìn)展[J];醫(yī)學(xué)綜述;2014年18期

8 王元芬;牛子儒;郭一帆;谷芳;劉忠宇;姚元慶;;人類(lèi)白細(xì)胞抗原G在宮頸癌組織中的表達(dá)及臨床意義[J];解放軍醫(yī)學(xué)院學(xué)報(bào);2014年07期

9 李觀(guān)貴;吳彤華;朱元昌;尹彪;宋成;陳曉燕;曾勇;林奇;;行輔助生殖技術(shù)患者流產(chǎn)胎兒人白細(xì)胞抗原-G基因14bp插入/缺失多態(tài)性分析[J];生殖醫(yī)學(xué)雜志;2014年02期

10 吳艷欣;王寧寧;謝洪哲;;HLA-G 14 bp基因多態(tài)性和子宮內(nèi)膜HLA-G表達(dá)水平與子宮內(nèi)膜異位癥的關(guān)系[J];新醫(yī)學(xué);2013年09期

相關(guān)碩士學(xué)位論文 前1條

1 楊冬梅;胚胎培養(yǎng)液中sHLA-G的表達(dá)與胚胎發(fā)育及種植的關(guān)系[D];廣西醫(yī)科大學(xué);2007年

，

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