本文選題：龍眼 + DCL基因　； 參考：《西北植物學(xué)報(bào)》2017年10期

【摘要】：為了解龍眼DCL基因的功能,該試驗(yàn)對(duì)龍眼基因組數(shù)據(jù)提取的DlDCL1、DlDCL2、DlDCL3和DlDCL4基因序列進(jìn)行啟動(dòng)子順式作用元件及其受miRNA調(diào)控的分析;并以龍眼胚性愈傷組織為材料,研究了DlDCLs不同基因成員在非生物脅迫和外源激素處理下的表達(dá)情況。結(jié)果顯示:(1)龍眼DCL基因啟動(dòng)子中除了TATA和CAAT外,還具有大量的光反應(yīng)元件、激素應(yīng)答元件、脅迫響應(yīng)元件、組織特異性調(diào)控元件及植物生長發(fā)育相關(guān)的順式調(diào)控元件,提示龍眼DCL基因啟動(dòng)子轉(zhuǎn)錄活性可能受到光、激素信號(hào)及逆境脅迫因素的誘導(dǎo)。(2)對(duì)調(diào)控龍眼DCL基因的miRNA進(jìn)行篩選,結(jié)果顯示DlDCL1受miR162和miR1024調(diào)控,DlDCL4受miR390和miR396調(diào)控。(3)實(shí)時(shí)熒光定量PCR顯示,在一定濃度范圍內(nèi),外源激素GA3、ABA和ETH均能下調(diào)DlDCLs基因的表達(dá),而高濃度ETH處理則顯著上調(diào)DlDCLs的表達(dá)。(4)高濃度蔗糖(6%)處理時(shí)DlDCL2、DlDCL3和DlDCL4顯著上調(diào)表達(dá),而低濃度(0.1%)處理時(shí)DlDCL1顯著上調(diào)表達(dá);不同溫度處理下,DlDCL1在34℃時(shí)顯著上升,DlDCL3隨著溫度的提高相對(duì)表達(dá)量逐漸減低;而DlDCL2和DlDCL4表達(dá)量差異不明顯;NaCl脅迫處理下,DlDCLs在1h處理時(shí)表達(dá)量下調(diào),但在其他不同時(shí)間點(diǎn)則上調(diào)表達(dá)。研究表明,龍眼DCL基因在外源激素及非生物脅迫處理下,并非是簡單的一對(duì)一響應(yīng),而是存在較為復(fù)雜的響應(yīng)機(jī)制。
[Abstract]:In order to understand the function of longan DCL gene, the promoter cis-acting elements of DlDCL1, DlDCL2, DlDCL3 and DlDCL3 gene sequences extracted from longan genome data and their regulation by miRNA were analyzed, and the embryogenic callus of longan was used as a material. The expression of different gene members of DlDCLs under abiotic stress and exogenous hormone treatment was studied. The results showed that in addition to TATA and CAAT, the promoter of longan DCL gene also had a large number of photoresponse elements, hormone response elements, stress response elements, tissue specific regulatory elements and cis-regulatory elements related to plant growth and development. The results suggested that the transcriptional activity of the promoter of longan DCL gene might be induced by light, hormone signal and stress factors. The results showed that DlDCL1 was regulated by miR162 and miR1024. DlDCL4 was regulated by miR390 and miR396. DlDCL2DlDCL3 and DlDCL4 were upregulated significantly in high concentration ETH treatment, while DlDCL1 expression was significantly up-regulated in low concentration (0.1%) treatment with DlDCL2DlDCL3 and DlDCL3 with high sucrose concentration. The relative expression of DlDCL3 decreased with the increase of temperature at 34 鈩，

本文編號(hào)：1800588