本文選題：羽葉三七 + 轉(zhuǎn)錄組測序��； 參考：《基因組學(xué)與應(yīng)用生物學(xué)》2017年06期

【摘要】：羽葉三七是五加科人參屬的名貴藥材,三萜皂苷為羽葉三七最主要的活性成分。為了探索羽葉三七根莖中皂苷物質(zhì)生物合成的分子基礎(chǔ),采用Illumina Hi Seq 2000高通量測序獲得羽葉三七根莖的轉(zhuǎn)錄組數(shù)據(jù);使用Trinity和TGICL軟件實(shí)現(xiàn)Uni Gene的de novo拼接;基于BLAST完成Uni Gene的蛋白功能注釋、KOG功能注釋、GO分類和KEGG代謝通路分析。最終通過de novo拼接注釋得到Uni Gene 62 240個。研究發(fā)現(xiàn),羽葉三七根莖部表達(dá)的26個Uni Gene與三萜碳環(huán)骨架合成相關(guān);三萜合成通路中的關(guān)鍵酶FPS、SS、SE等,分別有11 114個Uni Gene。該研究發(fā)現(xiàn)的三萜皂苷合成相關(guān)候選基因?qū)τ陉U明羽葉三七三萜皂苷合成方式研究提供了理論基礎(chǔ)。
[Abstract]:Panax notoginseng is a valuable medicinal material of Panax ginseng, and triterpenoid saponins are the main active components of Panax notoginseng. In order to explore the molecular basis of saponin biosynthesis in the rhizome of Panax notoginseng, Illumina Hi Seq 2000 high-throughput sequencing was used to obtain the transcriptional data of the rhizome of Panax notoginseng, and the de novo splicing of Uni Gene was realized by Trinity and TGICL software. BLAST was used to complete the functional annotation of Uni Gene: go classification and KEGG metabolic pathway analysis. Finally, Uni Gene 62,240 were obtained by de novo splicing annotation. It was found that 26 Uni Gene expressed in rhizome of Panax notoginseng were related to the synthesis of triterpenoid carbon cytoskeleton, and 11 114 Uni genes were found to be the key enzymes in triterpene synthesis pathway. The candidate genes related to triterpenoid saponins biosynthesis provided a theoretical basis for elucidating the synthesis of triterpenoid saponins in leaves.
【作者單位】： 武漢輕工大學(xué)生物與制藥工程學(xué)院;
【基金】：國家自然科學(xué)基金(No.81274023)資助
【分類號】：Q943.2;S567.5
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本文編號：1781741