本文選題：sCD40L + PTX3　； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:1、探討人外周血可溶性CD40配體(soluble CD40 ligand,s CD40L)、核因子-κB(nuclear factor-κB,NF-κB)及人五聚素3(pentraxin 3,PTX3)表達(dá)水平與腦梗死(cerebral infarction,CI)的關(guān)系。并探究人外周血s CD40L、NF-κB、PTX3三種炎癥因子之間的關(guān)聯(lián)。2、觀察CD40基因rs1535045單核苷酸多態(tài)性(Single Nucleotide Polymorphisms,SNP)與s CD40L、NF-κB及PTX3之間的關(guān)系,探究rs1535045 SNP在動(dòng)脈硬化形成過(guò)程中可能存在的炎癥信號(hào)通路。方法:在取得知情同意、符合倫理學(xué)的標(biāo)準(zhǔn)情況下,選取急性期腦梗死患者90例作為病例組,同時(shí)期體檢中心健康成年人85名作為對(duì)照組。收集血液標(biāo)本并提取外周血DNA,通過(guò)Taq Man探針技術(shù)確定rs1535045 SNP基因型,采用酶聯(lián)免疫吸附測(cè)定法(enzyme-linked immunosorbent assay,ELISA)檢測(cè)血漿s CD40L、PTX3濃度,采用流式細(xì)胞術(shù)(flow cytometry,FCM)檢測(cè)人外周血單個(gè)核細(xì)胞(peripheral blood mononuclear cells,PBMCs)中NF-κB p65的陽(yáng)性表達(dá)率。比較兩組間s CD40L、PTX3及NF-κB表達(dá)水平的差異,并分別分析此三種炎癥指標(biāo)兩兩之間的相關(guān)性,及與rs1535045 SNP之間的關(guān)系。結(jié)果:1、病例組血漿s CD40L濃度為(64.952±19.474)pg/m L,血漿PTX3濃度為(1.957±0.599)ng/m L,PBMCs中NF-κB陽(yáng)性率為(30.883±12.748)%;對(duì)照組血漿s CD40L濃度為(59.176±16.698)pg/m L,PTX3濃度為(1.737±0.632)ng/m L,PBMCs中NF-κB陽(yáng)性率為(18.082±8.674)%。病例組血漿s CD40L、PTX3濃度及PBMCs中NF-κB陽(yáng)性表達(dá)率均顯著高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。2、全部樣本中s CD40L血漿水平與NF-κB陽(yáng)性率之間存在正相關(guān)(r=0.485,p0.001),s CD40L與PTX3血漿水平之間存在正相關(guān)(r=0.251,P=0.001),NF-κB與PTX3表達(dá)水平之間存在正相關(guān)(r=0.301,P0.001)。分層分析病例亞組s CD40L血漿水平與NF-κB陽(yáng)性率之間存在正相關(guān)(r=0.764,P0.001),s CD40L與PTX3血漿水平之間存在正相關(guān)(r=0.445,P0.001),NF-κB與PTX3表達(dá)水平之間存在正相關(guān)(r=0.384,P0.001)。對(duì)照組亞中,尚不能認(rèn)為s CD40L血漿水平與NF-κB陽(yáng)性率之間存在相關(guān)性,P=0.716;尚不能認(rèn)為s CD40L與PTX3血漿水平存在相關(guān)性,P=0.867;尚不能認(rèn)為NF-κB陽(yáng)性表達(dá)率與PTX3血漿水平之間存在相關(guān)性,P=0.558。故尚不能認(rèn)為此3種炎癥因子兩兩之間存在相關(guān)性。3、CD40基因rs1535045 CC基因型、CT基因型、TT基因型的s CD40L濃度分別為(59.555±18.070)pg/ml、(63.453±17.193)pg/ml、(65.278±23.545)pg/ml,NF-κB p65陽(yáng)性率分別為(22.671±11.183)%、(25.365±12.287)%、(28.400±17.757)%,血漿PTX3濃度分別為(1.810±0.610)ng/ml、(1.863±0.609)ng/ml、(1.927±0.745)ng/ml,3種基因型組間差異均無(wú)統(tǒng)計(jì)學(xué)意義,但有濃度梯度。分層分析腦梗死病例組及對(duì)照組的3種基因型之間的血漿s CD40L濃度、PTX3濃度及NF-κB p65陽(yáng)性率的分布差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。進(jìn)一步分析病例組中動(dòng)脈粥樣硬化型腦梗死亞組的3種基因型在血漿s CD40L濃度、PTX3濃度及NF-κB p65陽(yáng)性率的分布差異仍無(wú)統(tǒng)計(jì)學(xué)意義(P0.05),但亦存在較明顯的濃度梯度。結(jié)論:1.急性期腦梗死患者體內(nèi)s CD40L、PTX3血漿濃度及NF-κB陽(yáng)性率均顯著升高,且此三種炎性因子兩兩之間均呈正相關(guān)。2.尚不能認(rèn)為rs1535045 SNP與s CD40L、NF-κB及PTX3表達(dá)水平之間存在關(guān)系。
[Abstract]:Objective: 1, to explore the relationship between human peripheral blood soluble CD40 ligand (soluble CD40 ligand, s CD40L), nuclear factor kappa B (nuclear factor- kappa B, NF- kappa B) and human five poly (3), and to explore the correlation between human peripheral blood and three kinds of inflammatory factors. The relationship between the rs1535045 single nucleotide polymorphism (Single Nucleotide Polymorphisms, SNP) and s CD40L, NF- kappa B and PTX3 to explore the possible inflammatory signaling pathway in the formation of arteriosclerosis by rs1535045 SNP. Methods: 90 cases of acute cerebral infarction were selected as the standard of informed consent and ethical standards. In the case group, 85 healthy adults at the same time physical examination center were used as the control group. The blood samples were collected and the peripheral blood DNA was extracted. The rs1535045 SNP genotype was determined by the Taq Man probe technique. The s CD40L of the plasma was detected by the enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA), and the PTX3 concentration was carried out by flow cytometry. The positive rate of NF- kappa B p65 in human peripheral blood mononuclear cells (peripheral blood mononuclear cells, PBMCs) was detected by try, FCM. The difference between the s CD40L and the expression level of the two groups was compared, and the correlation between the three inflammatory indices 22 and the relationship between them were analyzed. The concentration of L was (64.952 + 19.474) pg/m L, the plasma PTX3 concentration was (1.957 + 0.599) ng/m L, the positive rate of NF- kappa B in PBMCs was (30.883 + 12.748)%, and s CD40L concentration in the control group was (59.176 + 16.698) pg/m, and the concentration was (1.737 + 0.632). The positive rate of the positive expression was significantly higher than that in the control group (P0.05).2. There was a positive correlation between the plasma level of s CD40L and the positive rate of NF- kappa B in all samples (r=0.485, p0.001), and there was a positive correlation between s CD40L and PTX3 plasma level. There was a positive correlation between the plasma level of s CD40L and the positive rate of NF- kappa B (r=0.764, P0.001), and there was a positive correlation between the level of s CD40L and PTX3 plasma level (r=0.445, P0.001), and there was a positive correlation between the NF- kappa and the expression level. In correlation, P=0.716, it is not yet believed that there is a correlation between s CD40L and PTX3 plasma levels, P=0.867, and there is no correlation between the positive expression rate of NF- kappa B and the plasma level of PTX3, and P=0.558. is not considered to be associated with the correlation of.3, CD40 gene, genotype, and genotype. The concentration of D40L was (59.555 + 18.070) pg/ml, (63.453 + 17.193) pg/ml, (65.278 + 23.545) pg/ml, and the positive rates of NF- kappa B p65 were (22.671 + 11.183)%, (25.365 + 12.287)%, (28.400 + 17.757)%, and the plasma PTX3 concentration was (1.810 +%) ng/ml and ng/ml, and there was no statistical difference between the genotypes. But there was a concentration gradient. There was no significant difference in plasma s CD40L concentration, PTX3 concentration and NF- kappa B p65 positive rate between 3 genotypes in cerebral infarction case group and control group (P0.05). Further analysis of the concentration of s CD40L, PTX3 concentration and NF- kappa in the 3 genotypes of atherosclerotic cerebral infarction subgroup in the case group There was no significant difference in the distribution of B p65 positive rates (P0.05), but there was also a significant concentration gradient. Conclusion: 1. the s CD40L, PTX3 plasma concentration and the positive rate of NF- kappa B in patients with acute cerebral infarction were significantly increased, and the positive correlation between the three inflammatory factors 22 was not considered as rs1535045 SNP and S. There is a relationship between the level of expression.

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R743.3

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本文編號(hào)：1780691