本文選題：COX- + WNT�。� 參考：《現(xiàn)代腫瘤醫(yī)學》2016年03期

【摘要】：目的:研究siRNA靶向抑制環(huán)氧合酶-2(COX-2)基因后對胃癌細胞BGC823增殖、凋亡及藥物敏感性的影響。方法:將COX-2 siRNA(siRNA-COX-2組)及negative control siRNA(siRNA-control組)序列轉(zhuǎn)入細胞BGC823中,檢測細胞增殖能力,觀察多西紫杉醇(docetaxel艾素)、奧沙利鉑(L-OHP)、5-氟尿嘧啶(5-FU)的藥物敏感性變化,及轉(zhuǎn)染前后BGC823細胞COX-2、β-catenin、Bcl-2 mRNA基因和蛋白的表達。結(jié)果:siRNA-COX-2組細胞在轉(zhuǎn)染后第四天開始增殖能力下降,與siRNA-control組差異存在明顯統(tǒng)計學意義(P0.05),而siRNA-control組細胞于BGC823組增殖能力差異無明顯統(tǒng)計學意義(P0.05)。MTT法檢測轉(zhuǎn)染后胃癌細胞對艾素、奧沙利鉑、5-氟尿嘧啶的IC50較轉(zhuǎn)染前藥物敏感性明顯增加。流式細胞術檢測轉(zhuǎn)染前后胃癌細胞凋亡率為(3.08%±0.27%vs 16.14%±1.89%,P0.05),轉(zhuǎn)染后凋亡明顯增加(P0.05)。qRT-PCR法檢測發(fā)現(xiàn)轉(zhuǎn)染后COX-2、β-catenin、Bcl-2 mRNA基因的表達明顯下降,Western blot檢測發(fā)現(xiàn),轉(zhuǎn)染后48小時COX-2蛋白表達達到最低,轉(zhuǎn)染后48小時β-catenin、Bcl-2蛋白表達明顯下降(P0.05)。結(jié)論:通過下調(diào)COX-2基因的表達,可有效抑制WNT信號通路的激活,同時有效降低Bcl-2基因的表達。抑制COX-2基因后可有效降低胃癌細胞BGC823的細胞增殖能力,可有效提高對艾素、奧沙利鉑、5-氟尿嘧啶的藥物敏感性,有效促進細胞凋亡。
[Abstract]:Aim: to study the effects of siRNA targeting cyclooxygenase-2 cyclooxygenase-2 (COX-2) gene on the proliferation, apoptosis and drug sensitivity of gastric cancer cell line BGC823. Methods: the COX-2 siRNA(siRNA-COX-2 group and the negative control siRNA(siRNA-control group were transferred into the cell BGC823 to detect the proliferative ability of the cells, and to observe the drug sensitivity of docetaxel, oxaliplatin and 5-fluorouracil in docetaxel, oxaliplatin, 5-fluorouracil (5-FU). And the expression of Bcl-2 mRNA gene and protein in BGC823 cells before and after transfection. Results after transfection, the proliferation ability of the cells in the fraction siRNA-COX-2 group began to decrease on the fourth day after transfection, which was significantly different from that in the siRNA-control group (P 0.05), but there was no significant difference between the siRNA-control group and the BGC823 group. The sensitivity of oxaliplatin 5-fluorouracil (5-fluorouracil) in IC50 was significantly higher than that before transfection. The apoptotic rate of gastric cancer cells was 3.08% 鹵0.27%vs 16.14% 鹵1.89 before and after transfection by flow cytometry. After transfection, the expression of COX-2 protein reached the lowest at 48 hours after transfection. At 48 hours after transfection, the expression of 尾 -catenin B 2 protein decreased significantly (P 0.05). Conclusion: by down-regulating the expression of COX-2 gene, the activation of WNT signaling pathway and the expression of Bcl-2 gene can be effectively inhibited. Inhibition of COX-2 gene can effectively reduce the proliferation of gastric cancer cell line BGC823, can effectively enhance the drug sensitivity to oxaliplatin 5-fluorouracil, and promote cell apoptosis.
【作者單位】： 蘭州軍區(qū)蘭州總醫(yī)院;膠州市人民醫(yī)院;
【基金】：國家科技部、財政部科技惠民計劃(編號:2012GS620101) 甘肅省科技廳科技重大專項資助項目(編號:2010GS04390)
【分類號】：R735.2

【共引文獻】

相關期刊論文 前4條

1 趙德根;王建校;劉旭;徐濤;顧永清;;小干擾RNA的研究進展[J];現(xiàn)代生物醫(yī)學進展;2014年04期

2 于建平;韓曉鵬;王t，

本文編號：1780585