本文選題：金柑 + 克隆�。� 參考：《分子植物育種》2016年03期

【摘要】：本研究以融安金柑為試材,采用同源克隆技術(shù)獲得成花素基因Fc FT1全長序列,并對該基因的生物信息學(xué)和遺傳進化關(guān)系進行了分析。利用q RT-PCR技術(shù),分析了Fc FT1在花發(fā)育期間不同花器官、不同組織,以及日周期中Fc FT1在花、莖、葉的表達情況。結(jié)果表明:Fc FT1基因完整開放閱讀框為531 bp,編碼177個氨基酸。Fc FT1編碼的蛋白具有單一而非常保守的PEBP結(jié)構(gòu)域;蛋白質(zhì)結(jié)構(gòu)比較不穩(wěn)定,為親水蛋白。系統(tǒng)進化樹表明Fc FT1與甜橙、溫州蜜柑和枳殼中的FT同源基因親緣關(guān)系最近。q RT-PCR結(jié)果顯示,在花發(fā)育期,花藥中Fc FT1表達量最高;日周期變化中,Fc FT1在花、莖、葉中的平均表達量差距不顯著,表達較穩(wěn)定。本研究結(jié)果為進一步揭示FT基因在金柑開花調(diào)控中的功能奠定了基礎(chǔ)。
[Abstract]:In this study, the full-length sequence of FC FT1 was obtained by using homologous cloning technique, and the bioinformatics and genetic evolution of the gene were analyzed. The expression of FC FT1 in flower, stem and leaf during flower development was analyzed by Q RT-PCR technique in different flower organs, tissues and daily cycle. The results showed that the complete open reading frame of the 1: FC FT1 gene was 531 BP, encoding 177 amino acids. FC FT1 encoded a single and very conserved PEBP domain, and the protein structure was unstable and hydrophilic. The phylogenetic tree showed that FC FT1 was closely related to FT homologous genes in orange, bergamot and Fructus Aurantii. The results showed that FC FT1 expression was the highest in anther at flowering stage, and FC FT1 was in flower and stem during daily cycle change. The difference of average expression in leaves was not significant and the expression was stable. The results of this study laid a foundation for further revealing the function of FT gene in the regulation of flowering.
【作者單位】： 廣西大學(xué)農(nóng)學(xué)院亞熱帶農(nóng)業(yè)生物資源保護與利用國家重點實驗室;
【基金】：國家自然科學(xué)基金項目(31460508) 國家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系廣西柑橘創(chuàng)新團隊首席專家項目(nycytxgxcxtd-02-06)共同資助
【分類號】：S666
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本文編號：1780322