本文選題：水稻 + 褐穗　； 參考：《核農(nóng)學(xué)報(bào)》2017年12期

【摘要】：為鑒定新的脆桿突變體,從秈型水稻恢復(fù)系縉恢10號(hào)的甲基磺酸乙酯(EMS)誘變體庫(kù)中鑒定了一個(gè)全生育期植株變脆且開(kāi)花后稻穗呈褐色的突變體,暫命名為脆稈褐穗突變體fb1,對(duì)其進(jìn)行了機(jī)械強(qiáng)度、光合色素含量、木質(zhì)素和纖維素含量等測(cè)定,并開(kāi)展了遺傳分析和基因定位等研究。結(jié)果表明,與野生型相比,fb1莖稈和葉片的最大載荷分別下降了43.3%和63.1%,莖稈的纖維素和木質(zhì)素含量分別為野生型的76.5%和66.6%,葉片的纖維素和木質(zhì)素含量則分別為野生型的91.0%和95.5%,均顯著降低。開(kāi)花前,fb1的穗部性狀與野生型相比無(wú)變化,開(kāi)花后穎殼逐漸褐化,成熟后呈灰褐色。fb1的葉綠素a、葉綠素b和類胡蘿卜素含量分別為野生型的74.8%、90.5%和85.3%,均呈顯著或極顯著下降。遺傳分析表明,該性狀受一對(duì)隱性核基因調(diào)控,基于西農(nóng)1A/fb1的F2群體,利用SSR等分子標(biāo)記最終將調(diào)控基因FB1精細(xì)定位在第1染色體SSR標(biāo)記RM1268和RM11669之間42 kb的物理范圍內(nèi),包含7個(gè)注釋基因。該研究結(jié)果不僅為FB1基因的圖位克隆奠定了基礎(chǔ),也為植物細(xì)胞壁發(fā)育分子機(jī)理研究和新能源水稻育種提供了材料。
[Abstract]:In order to identify a new brittleness mutant, a new mutant with brown spikes after flowering was identified from the mutant bank of Jinhui 10, an indica rice restorer line Jinhui 10. The mutant fb1 was tentatively named as Fb1. The mechanical strength, photosynthetic pigment content, lignin and cellulose content were determined, and genetic analysis and gene mapping were carried out. The results show that Compared with wild type, the maximum load of stem and leaf decreased by 43.3% and 63.1%, respectively. The contents of cellulose and lignin of stem were 76.5% and 66.6% of that of wild type, respectively, and the contents of cellulose and lignin in leaves were 91.0% and 95.5B of wild type, respectively. The panicle characters of Fb1 before anthesis had no change compared with the wild type. The glume was browning gradually after anthesis, and the content of chlorophyll a, chlorophyll b and carotenoid were 74.80.5% and 85.3% of that of the wild type, respectively, and the contents of chlorophyll b and carotenoid were significantly or extremely significantly lower than those of the wild type, and the contents of chlorophyll a, chlorophyll b and carotenoid were 74.80.5% and 85.3% of the wild type, respectively. Genetic analysis showed that the trait was regulated by a pair of recessive nuclear genes. Based on the F2 population of Xinong 1A/fb1, the regulatory gene FB1 was finely mapped within the physical range of 42 kb between RM1268 and RM11669 on chromosome 1 by using SSR and other molecular markers. Contains seven annotated genes. The results not only laid a foundation for the mapping cloning of FB1 gene, but also provided materials for the molecular mechanism of cell wall development in plants and the breeding of rice with new energy sources.
【作者單位】： 西南大學(xué)水稻研究所/轉(zhuǎn)基因植物與安全控制重慶市重點(diǎn)實(shí)驗(yàn)室;四川省農(nóng)業(yè)科學(xué)院水稻高粱研究所;
【基金】：國(guó)家自然科學(xué)基金(31171178) 中央高�；究蒲袠I(yè)務(wù)費(fèi)項(xiàng)目(XDJK2015C117)
【分類號(hào)】：S511

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