本文選題：基因編輯 + CRISPR-Cas��； 參考：《生命科學(xué)》2016年08期

【摘要】：高效、特異的目標(biāo)基因組位點(diǎn)修飾一直是基因工程研究的重點(diǎn)和挑戰(zhàn)。靶向基因編輯技術(shù)不僅能夠有效地用于建立動物和細(xì)胞疾病模型、培育動植物新品種,并具有治療諸多疾病的重大潛力。近年來靶向核酸酶技術(shù)的研究取得了重大進(jìn)展,且逐漸成為基因編輯的主流工具,特別是規(guī)律成簇間隔短回文重復(fù)序列(CRISPR-Cas9)技術(shù)因其靶向編輯目的基因的特異性、高效性和設(shè)計的簡便性等諸多優(yōu)點(diǎn),得到更為廣泛的應(yīng)用。在中國科學(xué)院干細(xì)胞先導(dǎo)專項的支持下,基因編輯技術(shù)攻關(guān)團(tuán)隊在進(jìn)一步改造和應(yīng)用CRISPR-Cas9技術(shù)方面取得了一系列成果,就此進(jìn)行全方面的總結(jié)。
[Abstract]:Efficient and specific target genomic site modification has been the focus and challenge of genetic engineering.Target gene editing technology can be used to establish animal and cell disease models and to cultivate new species of animal and plant effectively, and has great potential for the treatment of many diseases.In recent years, great progress has been made in the study of targeted nuclease technology, and has gradually become the mainstream tool of gene editing, especially the regular clustering short palindrome repeat sequence CRISPR-Cas9, because of the specificity of its target gene editing.High efficiency and design simplicity and many other advantages, has been more widely used.With the support of the stem cell pilot project of the Chinese Academy of Sciences, the key technology team of gene editing has made a series of achievements in the further modification and application of CRISPR-Cas9 technology, which is summarized in all aspects.
【作者單位】： 中國科學(xué)院動物研究所干細(xì)胞與生殖生物學(xué)國家重點(diǎn)實(shí)驗(yàn)室;中國科學(xué)院上海生命科學(xué)研究院生物化學(xué)與細(xì)胞生物學(xué)研究所;
【基金】：中國科學(xué)院“干細(xì)胞與再生醫(yī)學(xué)研究”戰(zhàn)略性先導(dǎo)科技專項(XDA01010409)
【分類號】：Q78
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本文編號：1773859