本文選題：紅細(xì)胞血型 + 試劑紅細(xì)胞　； 參考：《中國(guó)輸血雜志》2017年10期

【摘要】：目的建立重要的紅細(xì)胞血型的基因分型技術(shù)用于篩選抗原譜合理的試劑紅細(xì)胞。方法首先建立檢測(cè)紅細(xì)胞血型系統(tǒng)Rh CE、Kidd、MNS、Duffy、Diego、Lutheran、Colton、Lutheran和Kell等的聚合酶鏈?zhǔn)椒磻?yīng)-序列特異性引物(PCR-SSP)分型技術(shù);對(duì)于無法獲得相關(guān)血型試劑的Diego和Colton血型系統(tǒng),采用測(cè)序方法驗(yàn)證該技術(shù)檢測(cè)血型的準(zhǔn)確性,其他6個(gè)血型系統(tǒng)則用血清學(xué)方法驗(yàn)證該技術(shù)檢測(cè)血型的準(zhǔn)確性;最后應(yīng)用該方法對(duì)82名O型固定獻(xiàn)血者的8個(gè)血型系統(tǒng)做基因分型。結(jié)果血型基因PCR-SSP分型技術(shù)的結(jié)果,與血清學(xué)和測(cè)序檢測(cè)結(jié)果比較,完全一致。所建立的血型基因分型技術(shù)對(duì)82名O型獻(xiàn)血者8種紅細(xì)胞血型檢測(cè),篩選出13位可能適合提供試劑紅細(xì)胞的獻(xiàn)血者。結(jié)論成功建立了分型準(zhǔn)確、方便、經(jīng)濟(jì)的紅細(xì)胞血型基因PCR-SSP技術(shù),用于從大量獻(xiàn)血者中篩選出抗原譜合理的試劑紅細(xì)胞。
[Abstract]:Objective to establish an important genotyping technique of erythrocyte blood group for screening reagent erythrocytes with reasonable antigen spectrum.Methods the polymerase chain reaction-sequence specific primer (PCR-SSPP) typing technique was established for the detection of erythrocyte blood group system Rh CED MNS Duffy Diegol Lutheran and Kell, and the Diego and Colton blood group systems which could not obtain the relevant blood group reagents were used.The accuracy of this technique was verified by sequencing method, and the other 6 blood group systems were tested by serological method.Finally, 8 blood group systems of 82 O-type fixed blood donors were genotyped by this method.Results the results of PCR-SSP typing were consistent with those of serology and sequencing.The established genotyping technique was used to detect 8 kinds of erythrocyte blood groups of 82 O blood donors and 13 blood donors who might be suitable for providing reagent red blood cells were selected.Conclusion the accurate, convenient and economical PCR-SSP technique of erythrocyte blood group gene has been successfully established, which can be used to screen the red blood cells with reasonable antigen profile from a large number of blood donors.
【作者單位】： 成都軍區(qū)總醫(yī)院輸血科;中國(guó)醫(yī)學(xué)科學(xué)院輸血研究所;
【基金】：四川省科技廳科技計(jì)劃項(xiàng)目(2015JY0121)
【分類號(hào)】：R457.11
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本文編號(hào)：1752437