本文選題：葡萄 + CIPK��； 參考：《園藝學(xué)報(bào)》2017年08期

【摘要】：以歐洲葡萄‘佳麗釀’(Carinena)為材料,利用RT-PCR方法獲得兩個(gè)CIPK基因的全長(zhǎng)cDNA序列,分別為VvCIPK13和VvCIPK14。VvCIPK13全長(zhǎng)為1 501 bp,包括一個(gè)1 395 bp的ORF,編碼465個(gè)氨基酸;VvCIPK14全長(zhǎng)為1 616 bp,包括一個(gè)1 404 bp的ORF,編碼468個(gè)氨基酸。生物信息學(xué)分析表明兩個(gè)蛋白均含有兩個(gè)保守結(jié)構(gòu)域:絲氨酸/蘇氨酸激酶結(jié)構(gòu)域和NAF結(jié)構(gòu)域,兩個(gè)結(jié)構(gòu)域之間有連接域,但VvCIPK13和VvCIPK14的同源性較低,僅有40.33%。利用熒光定量PCR技術(shù)研究VvCIPK13和VvCIPK14在葡萄不同組織、植物生長(zhǎng)調(diào)節(jié)劑誘導(dǎo)和逆境脅迫下的表達(dá)模式,結(jié)果表明:VvCIPK13和VvCIPK14表達(dá)具有組織特異性,均在卷須中高表達(dá);VvCIPK13對(duì)6-BA誘導(dǎo)有響應(yīng),而VvCIPK14對(duì)6-BA、ABA、IAA、SA、MeJA和GA_3等誘導(dǎo)均有不同程度的響應(yīng);VvCIPK13和VvCIPK14均響應(yīng)高鹽和干旱,但不響應(yīng)低溫,其中VvCIPK14的響應(yīng)最為強(qiáng)烈。推測(cè)VvCIPK13和VvCIPK14在葡萄的非生物脅迫過程中發(fā)揮重要的作用。
[Abstract]:The full-length cDNA sequences of two CIPK genes were obtained by RT-PCR method from Carinena, a European grape.The total length of VvCIPK13 and VvCIPK14.VvCIPK13 were 1 501 BP, including an ORF of 1 395 BP and a full length of 1 616 BP encoding 465 amino acids VvCIPK14, including an ORF of 1 404 BP, encoding 468 amino acids.Bioinformatics analysis showed that the two proteins contained two conserved domains: serine / threonine kinase domain and NAF domain. The two domains were linked to each other, but the homology between VvCIPK13 and VvCIPK14 was low (40.33).The expression patterns of VvCIPK13 and VvCIPK14 in different tissues of grape, plant growth regulator induction and stress stress were studied by fluorescence quantitative PCR. The results showed that the expression of VvCIPK13 and VvCIPK14 in tendrils were tissue-specific, and the high expression of VvCIPK13 in tendrils was responsive to 6-BA induction.However, VvCIPK14 had different responses to the induction of 6-BAA AIAA, SAA, Meja and GA_3, and both VvCIPK13 and VvCIPK14 responded to high salinity and drought, but not to low temperature, among which VvCIPK14 had the strongest response.It is speculated that VvCIPK13 and VvCIPK14 play an important role in abiotic stress of grape.
【作者單位】： 河南科技學(xué)院園藝園林學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(U1404321) 河南科技學(xué)院高層次人才科研項(xiàng)目(12008001)
【分類號(hào)】：S663.1
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本文編號(hào)：1751463