發(fā)布時間：2018-04-14 19:47

  本文選題：基因芯片 + 分枝桿菌��； 參考：《中國人獸共患病學報》2017年08期

【摘要】：目的探討基因芯片技術檢測分枝桿菌和異煙肼(INH)、利福平(RFP)耐藥性在結核性膿胸診斷中的臨床應用價值。方法收集2011年1月至2015年12月臨床疑似結核性膿胸患者182例的膿液標本,分別應用基因芯片技術和快速培養(yǎng)法進行檢測,比較兩種方法的特異性和敏感性;同時選取36例臨床確診為結核性膿胸患者,且基因芯片法和MGIT培養(yǎng)法均為陽性的標本進行INH、RFP耐藥性檢測。以MGIT培養(yǎng)法的藥敏結果為標準,評價基因芯片法的靈敏度、特異度和符合率。結果 182例疑似結核性膿胸患者中,135例臨床確診為結核性膿胸,基因芯片法和快速培養(yǎng)法的特異性均為95.7%(45/47),靈敏度分別為48.9%(66/135)和26.7%(36/135),二者比較有統(tǒng)計學差異(χ2=80.5,P0.05)�；蛐酒z測RFP耐藥性靈敏度、特異度和符合率均為100%;而檢測INH耐藥性的靈敏度、特異度和符合率分別為50.0%(1/2)和97.1%(33/34)和94.4%。結論基因芯片技術檢測分枝桿菌具有較高的、特異性,可快速鑒別出非結核分枝桿菌,且可有效檢測結核分枝桿菌異煙肼和利福平的耐藥性,對結核性膿胸的早期診斷與治療有重要意義。
[Abstract]:Objective to investigate the clinical application value of gene chip technique in the diagnosis of tuberculous empyema by detecting the resistance of Mycobacterium isoniazide (INHN) and rifampicin (rifampicin) in the diagnosis of tuberculous empyema.Methods 182 patients with suspected tuberculous empyema were collected from January 2011 to December 2015. The specificity and sensitivity of the two methods were compared by gene chip technique and rapid culture method.At the same time, 36 patients with tuberculous empyema were tested for drug resistance by gene chip method and MGIT culture method.The sensitivity, specificity and coincidence rate of gene chip assay were evaluated according to the drug sensitivity of MGIT culture method.Results among 182 suspected tuberculous empyema patients, 135 cases were clinically diagnosed as tuberculous empyema. The specificity of gene chip method and rapid culture method were 95.745 / 47, the sensitivity was 48.9% / 135) and 26.775% 36 / 135% respectively. There was a statistical difference between the two methods (蠂 2 80.5% P 0.05).The sensitivity, specificity and coincidence rate of RFP resistance detected by gene chip method were 100, while the sensitivity, specificity and coincidence rate of detecting INH resistance were 50.01 / 2) and 97.1g / 34) and 94.4%, respectively.Conclusion the detection of Mycobacterium tuberculosis by gene chip technique has a high specificity and can be used to identify non-tuberculous mycobacteria quickly and to detect the resistance of isoniazid and rifampicin to Mycobacterium tuberculosis.It is important for the early diagnosis and treatment of tuberculous empyema.
【作者單位】： 福建醫(yī)科大學福州總醫(yī)院臨床醫(yī)學院呼吸與危重癥醫(yī)學科;福建中醫(yī)藥大學附屬第三人民醫(yī)院;福建醫(yī)科大學教學醫(yī)院福州肺科醫(yī)院檢驗科;福建省疾病預防控制中心;福建醫(yī)科大學教學醫(yī)院福州肺科醫(yī)院結核科;
【基金】：福建省衛(wèi)生和計劃生育委員會青年科研課題(No.2014-1-74)~~
【分類號】：R521.7

【相似文獻】

相關期刊論文 前10條

1 楊立生,劉維民,黨潤民,馬永金;結核性膿胸并發(fā)癥的影像學診斷[J];第四軍醫(yī)大學吉林軍醫(yī)學院學報;2002年03期

2 趙艷民;結核性膿胸的治療[J];河南醫(yī)藥信息;2003年02期

3 嚴曉峰;曹培明;;兩種方法治療結核性膿胸療效比較[J];現代醫(yī)藥衛(wèi)生;2006年06期

4 龐玉嬋;;肺結核合并結核性膿胸一例[J];山西醫(yī)藥雜志(下半月刊);2008年01期

5 楊鳳;白云;鄭浩;魏素霞;;結核性膿胸術前肺功能分析[J];河北醫(yī)藥;2010年24期

6 宋育,趙健,李森,夏有祿;成人結核性膿胸的外科治療[J];江蘇醫(yī)藥;1995年03期

7 林明華;結核性膿胸232例分析[J];海峽預防醫(yī)學雜志;1996年04期

8 于清言,曲麗杰,楊玉;結核性膿胸沖洗及用藥治療10例觀察[J];齊齊哈爾醫(yī)學院學報;2002年06期

9 王淑娟;68例急性結核性膿胸內科治療探討[J];現代醫(yī)藥衛(wèi)生;2005年08期

10 李任飛;;，

本文編號：1750759