本文選題：桉樹 + 耐寒性��； 參考：《分子植物育種》2017年02期

【摘要】：為了探討過氧化物酶(Peroxidase,POD)活性、同工酶及基因表達(dá)變化與桉樹耐寒性間的相關(guān)性,選用耐寒性分別為弱、中、強(qiáng)的韋鄧桉、鄧恩桉和湘鄧桉半年苗齡樹苗作材料,先26℃培養(yǎng)兩周,再5℃冷處理24 h后,剪取葉片檢測(cè)3種桉樹POD活性,同工酶酶譜和4個(gè)POD基因的轉(zhuǎn)錄變化。韋鄧桉冷處理前后的POD活性無(wú)顯著性差異,但冷處理后,分子量較大的同工酶表達(dá)量降低,分子量較小的同工酶表達(dá)量升高。冷處理后,鄧恩桉POD活性顯著降低,各條同工酶活性均有下降;湘鄧桉POD活性升高明顯,除L4和L5含量略有下降外,其他酶帶活性均有增加。設(shè)26℃下相同樹種的同種基因表達(dá)量為1,冷處理后,韋鄧桉的POD1和POD2的表達(dá)略有下降,POD3和POD4的表達(dá)量分別升高到常溫下的1.31和1.92倍;鄧恩桉的POD1表達(dá)量沒有改變,但POD2、POD3和POD4的表達(dá)量分別降低到常溫下的0.44、0.66和0.66倍;湘鄧桉的POD1、POD2、POD3和POD4的表達(dá)均有顯著降低,分別為常溫下的0.79、0.29、0.19和0.20倍。結(jié)果表明:冷處理后,POD2、POD3和POD4基因的表達(dá)變化與桉樹抗寒性呈負(fù)相關(guān)關(guān)系。本研究可為桉樹耐寒品種的選育提供參考。
[Abstract]:In order to investigate the peroxidase (Peroxidase, POD) activity, isozyme and gene expression changes and correlation between the cold resistance of Eucalyptus, the cold resistance were weak, strong Wei Deng Deng Enan and Xiang Deng eucalyptus, eucalyptus seedlings seedlings as materials for half a year, the first 26 C were cultured for two weeks and then 5 degrees cold treatment for 24 h after the activity of 3 kinds of Eucalyptus Leaf clipping POD detection, transcriptional changes of Isozyme and 4 POD genes. There was no significant difference before and after the Wei Dengan cold treatment POD activity, but after cold treatment, the expression of Isozyme of higher molecular weight decreased, isozyme of smaller molecular weight. The expression level increased after cold treatment. Deng Enan POD activity decreased significantly, the enzyme activity decreased; POD activity increased significantly in Hunan Dunn eucalyptus, except L4 and L5 content decreased slightly, other enzyme activity were increased. The same gene set the same species under 26 DEG C expression was 1, after cold treatment, Wei Deng Eucalyptus PO The expression of D1 and POD2 decreased slightly, expression of POD3 and POD4 were increased to 1.31 and 1.92 times at room temperature; Deng Enan POD1 expression did not change, but the expression of POD2, POD3 and POD4 were reduced to normal temperature 0.44,0.66 and 0.66 times; Xiang Deng Eucalyptus POD1, POD2, expressed in POD3 and POD4 decreased significantly, respectively at room temperature 0.79,0.29,0.19 and 0.20 times. The results showed that: after cold treatment, POD2, POD3 and POD4 gene expression and cold resistance of Eucalyptus was negatively correlated. This research can provide reference for the breeding of cold resistant Eucalyptus varieties.

【作者單位】： 嶺南師范學(xué)院生命科學(xué)與技術(shù)學(xué)院;嶺南師范學(xué)院資源植物工程中心;
【基金】：國(guó)家自然科學(xué)基金(31570660) 廣東省自然科學(xué)基金(2014A030307005;2015A030313560;2016A030313668) 湛江市科研項(xiàng)目(2014A03014)共同資助
【分類號(hào)】：S792.39

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