本文選題：α-　切入點：半乳糖苷轉(zhuǎn)移酶基因(GGTA)　出處：《農(nóng)業(yè)生物技術(shù)學報》2017年10期

【摘要】：用于異種器官移植供體研究的α-1,3半乳糖苷轉(zhuǎn)移酶基因(α-1,3-galactosyltransferase,GGTA1)敲除豬(Sus scrofa)的繁育性能和健康狀態(tài)是異種器官移植之路能否成功的前提。為了觀察本課題組制備的GGTA1基因敲除(GGTA1 knockout,GTKO)巴馬小型豬(Bama minipig,BMP)的遺傳基因型、窩產(chǎn)仔數(shù)及其血常規(guī)和血生化指標變化,評估其繁殖和健康狀況,本研究對其進行定向培育,并繁育到F3代,通過PCR產(chǎn)物測序鑒定仔豬GGTA1基因敲除類型,異硫氰酸熒光素標記的植物凝集素(fluorescein isothiocyanate-griffonia simplicifolia isolectin B4,FITC-GSIB4)與仔豬外周血單個核細胞(peripheral blood mononuclear cells,PBMC)孵育后流式細胞術(shù)檢測α-1,3半乳糖苷(α-1,3-galactosidase,α-1,3-Gal)的表達,以窩產(chǎn)仔數(shù)測定繁殖力,以血常規(guī)和血生化檢測反映健康狀況。結(jié)果顯示,GGTA1基因的遺傳符合孟德爾分離定律;GGTA1敲除純合子(GGTA~(1-/-))仔豬的流式檢測熒光強度低;GGTA~(1-/-)巴馬豬母豬頭胎窩產(chǎn)仔數(shù)為7.33±1.70頭,經(jīng)產(chǎn)母豬窩產(chǎn)仔數(shù)為9.43±1.68頭,與已有報道的普通巴馬小型豬繁殖力無明顯差異;血常規(guī)和血生化檢測的各項指標與普通巴馬豬基本無差異。本研究建立了GTKO巴馬小型豬家系,GTKO巴馬小型豬遺傳穩(wěn)定,繁殖力正常,生理健康,GGTA~(1-/-)巴馬小型豬有效解決了超急性排斥反應。研究結(jié)果為異種器官移植研究提供了良好供體
[Abstract]:The reproductive performance and health status of 偽 -1C 3-galactosyltransferase (GGTA1) knockout of Sus scrofaa for xenotransplantation is the prerequisite for the success of xenotransplantation.In order to observe the genetic genotype, litter size, blood routine and biochemical indexes of GGTA1 gene knockout GGTA1 knockout GTKOO (Bama miniature piglet) prepared by our team, and to evaluate its reproductive and health status, the aim of this study was to investigate the genetic genotype, litter size, blood routine and blood biochemical indexes, and to evaluate their reproductive and health status.The PCR products were sequenced to identify the GGTA1 gene knockout type of piglets.寮傜～姘伴吀鑽у厜绱犳爣璁扮殑妞嶇墿鍑濋泦绱，

本文編號：1711066