本文選題：EA激活基因阻遏子　切入點：動脈粥樣硬化　出處：《心臟雜志》2016年01期

【摘要】：目的探討E1A激活基因阻遏子(CREG)在動脈粥樣硬化(AS)血管中的表達(dá)及其與炎癥因子的關(guān)系。方法 Apo E(-/-)小鼠6周齡斷奶后給予高脂喂養(yǎng)。8周后取主動脈血管,采用HE染色觀察血管的形態(tài)學(xué)變化;采用免疫熒光染色觀察CREG、SMα-actin和巨噬細(xì)胞標(biāo)志物Mac-3的表達(dá)變化。取高脂喂養(yǎng)的1~8周小鼠血管,采用Western blot方法檢測AS血管中CREG及核轉(zhuǎn)錄因子(NF)-κB的表達(dá)的變化。結(jié)果在Apo E(-/-)小鼠高脂喂養(yǎng)8周,主動脈血管AS斑塊明顯形成,斑塊內(nèi)有膽固醇結(jié)晶,斑塊凸凹不平,管腔明顯狹窄。免疫熒光顯示AS血管中,CREG表達(dá)明顯下降,同時SMα-actin表達(dá)也下降,而Mac-3表達(dá)增高。Apo E(-/-)小鼠高脂喂養(yǎng)2~8周,取動脈血管行蛋白定量分析,結(jié)果顯示高脂喂養(yǎng)2周時,CREG在動脈血管中的表達(dá)不是降低,而是明顯升高,高脂喂養(yǎng)第4周,CREG表達(dá)急劇下降,而后又逐漸上升,但不能升至正常水平。同時NF-κB隨著時間的推移,表達(dá)逐漸升高。結(jié)論在AS進(jìn)程中,血管中膜的VSMCs由收縮表型向合成表型轉(zhuǎn)化,細(xì)胞增生活躍、分化減弱,CREG作為維持VSMCs分化的蛋白,在血管中的表達(dá)與AS進(jìn)展密切相關(guān),同時伴隨著炎癥因子表達(dá)逐漸升高。
[Abstract]:Objective to investigate the expression of E 1A activated gene repressor (CREGG) in atherosclerotic artery (ASA) and its relationship with inflammatory factors.Methods Aortic vessels were taken from Apo EU-R mice after weaning at 6 weeks of age and fed with high fat for 8 weeks. The morphological changes of aorta were observed by HE staining, and the expression of CREGSM 偽 -actin and macrophage marker Mac-3 were observed by immunofluorescence staining.Western blot method was used to detect the expression of CREG and nuclear transcription factor NF-kappa B in the blood vessels of mice fed with high fat for 1 to 8 weeks.Results after 8 weeks of hyperlipidemia feeding, atherosclerotic atherosclerotic plaques in the aorta were found in Apo E- / R mice. Cholesterol crystals were found in the plaques, the plaques were uneven and the lumen was obviously narrow.Immunofluorescence showed that the expression of creg and SM 偽 -actin were significantly decreased in as blood vessel, and the expression of SM 偽 -actin was also decreased, while the expression of Mac-3 was increased in mice fed with high fat for 2 weeks.The results showed that the expression of CREG in arterial vessels was not decreased, but increased significantly at the 2nd week of high fat feeding. At the 4th week of high fat feeding, the expression of CREG decreased sharply, then increased gradually, but could not reach the normal level.At the same time, the expression of NF- 魏 B increased with time.Conclusion in the process of as, the VSMCs of vascular mesenchymal membrane is transformed from contractile phenotype to synthetic phenotype, cell proliferation is active, differentiation is weakened and creg is the protein to maintain differentiation of VSMCs. The expression of CREG in blood vessel is closely related to the progression of as.At the same time, the expression of inflammatory factors gradually increased.
【作者單位】： 沈陽軍區(qū)總醫(yī)院心內(nèi)科;
【基金】：遼寧省博士啟動基金資助(20131136)
【分類號】：R543.5
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本文編號：1710821