本文選題：大豆　切入點：Gm　出處：《生物技術通報》2017年08期

【摘要】：以大豆天隆1號為材料提取基因組DNA,分別克隆到長2 631 bp的Gm COL1啟動子和2 809 bp的Gm COL13啟動子。此外,還克隆到Gm COL1和Gm COL13基因組基因長度分別為3 488 bp和2 798 bp,它們分別編碼含348個和366個氨基酸的蛋白質。利用Plant CARE分析Gm COL1和Gm COL13的啟動子序列發(fā)現(xiàn),它們均含有以下調控元件:生物鐘元件(Circadian)、光響應元件(ACEG-box等)、ABA響應元件(ABRE)、干旱誘導元件(MBS)、低溫響應元件(LTR)。Prot Param分析表明,Gm COL1和Gm COL13蛋白質的分子量分別為38.47 k D和40.91 k D,并且都是親水性蛋白。GOR分析顯示,Gm COL1和Gm COL13的二級結構含有Alpha helix,Extended strand和Random coil。在線網(wǎng)站PROSITE的分析說明,Gm COL1和Gm COL13有兩個Zinc finger B-box和一個CCT domain(CO,CO-LIKE and TOC1 domain)結構域。
[Abstract]:Genomic DNA was extracted from soybean Tianlong 1 and cloned into 2 631 BP GM COL1 promoter and 2 809 BP GM COL13 promoter, respectively. The genomic gene length of GM COL1 and GM COL13 were 3 488 BP and 2 798 BP, respectively, which encoded 348 and 366 amino acids, respectively. The promoter sequences of GM COL1 and GM COL13 were analyzed by Plant CARE. They all contain the following regulatory elements: circadian clock element, light response element ACEG-box, ACEG-box, drought induced element, and low temperature response element, LTR.Prot Param analysis shows that the molecular weight of Gm COL1 and GM COL13 protein are 38.47kD and 40.91, respectively. The molecular weight of Gm COL1 and GM COL13 protein are 38.47kD and 40.91, respectively. The molecular weight of Gm COL1 and GM COL13 protein are 38.47kD and 40.91, respectively. The secondary structures of Gm COL1 and GM COL13 contain Alpha helix extended strand and Random Coil.The analysis of PROSITE shows that Gm COL1 and GM COL13 have two Zinc finger B-box and one CCT domaine CO-CO-LIKE and TOC1 domain.
【作者單位】： 中國農(nóng)業(yè)科學院作物科學研究所國家農(nóng)作物基因資源與遺傳改良重大科學工程農(nóng)業(yè)部北京大豆生物學重點實驗室;
【基金】：國家自然科學基金項目(31371703,31570289) 轉基因專項(2016ZX08004-005,2016ZX08009001-001)
【分類號】：Q943.2;S565.1
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本文編號：1690381